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Movie 1. Dynamic view of catalysis by the Sir2 protein from yeast, yHst2. The movie depicts the three reaction states of the ternary yHst2/acetyllysine histone H4/NAD⁺ analogue complexes, showing the substrate analogue complex (with carba-NAD⁺), the intermediate analogue complex (with ADP-ribose), and the product complex (with 2'-O-acetyl-ADP-ribose). The color-coding is as follows: small protein domain, dark blue; large protein domain, light blue; β1–α2 protein loop, purple; catalytic Asn-116 and His-135 residues, red; NAD⁺ analogue, yellow; and acetyllysine histone H4, green.