Supporting Information

Files in this Data Supplement:

Movie 1. Video clip of a granule containing neuropeptide Y (NPY)-enhanced GFP (EGFP) (Upper) and FM4-64 (Lower) fusing with the plasma membrane as described in Fig. 2A. Each frame of the movie is 30 ms.

Movie 2. Video clip of FM4-64 spreading into the plasma membrane during exocytosis as described in Fig. 3B. Each frame of the movie is 30 ms.

Movie 3. Video clip of an EGFP-phogrin-labeled granule that brightens during exocytosis, dims, and then rebrightens during superfusion with 50 mM NH₄Cl as described in Fig. 4A. Each frame of the movie is 0.5 s.