Organism Appendix S7.Results ofANCOVA's across species,testing the effectcentromeric vs.telomeric location (location),the total number ofcoding base-pairs (Exon),the total number ofbase pairs in introns between coding exons (Intron),and their interactions.One transcriptper gene was chosen randomly.In all species exceptopossum,X-linked genes had significantly lower codon usage bias compared to autosomal genes.