Supplemental Data. Tulin and Cross. (2014). Plant Cell 10.1105/tpc.114.129312 CDK sequence alignment CLUSTAL CrCDKA/1-327 ------------MDKYEKLERAGEGTYGVVFKARDRYTN---EIVALKKIRLEQ---EDE CrCDKB/1-325 ------------MDAYEKIEKIGEGTYGKVYKARDINTG---KLVALKKCRLEM---EEE HsCDK1/1-297 ------------MEDYTKIEKIGEGTYGVVYKGRHKTTG---QVVAMKKIRLES---EEE MmCDK1/1-297 ------------MEDYIKIEKIGEGTYGVVYKGRHRVTG---QIVAMKKIRLES---EEE AtCDKA-1/1-294 ------------MDQYEKVEKIGEGTYGVVYKARDKVTN---ETIALKKIRLEQ---EDE SpCdc2/1-297 ------------MENYQKVEKIGEGTYGVVYKARHKLSG---RIVAMKKIRLED---ESE ScCdc28/1-298 --------MSGELANYKRLEKVGEGTYGVVYKALDLRPGQGQRVVALKKIRLES---EDE OtCDKA/1-296 ------------MENYEKVAKIGEGTYGVVYKAKSLRDD---AMVALKRIRLDQ---DDE AtCDKB2-2/1-315 MDNNGVKPAVSAMEAFEKLEKVGEGTYGKVYRAREKATG---MIVALKKTRLHE---DEE AtCDKB2-1/1-313 MDEG--VIAVSAMDAFEKLEKVGEGTYGKVYRAREKATG---KIVALKKTRLHE---DEE AtCDKB1-2/1-311 ------------MEKYEKLEKVGEGTYGKVYKAMEKTTG---KLVALKKTRLEM---DEE OtCDKB/1-329 ------MGGHSINDAYEKLEKIGQGTYGKVYKARDRATG---RLVALKKTRLEM---EEE AtCDKB1-1/1-309 ------------MEKYEKLEKVGEGTYGKVYKAMEKGTG---KLVALKKTRLEM---DEE gi|4502743|ref|NP_001790.1|/1-346 ----MALDVKSRAKRYEKLDFLGEGQFATVYKARDKNTN---QIVAIKKIKLGHRSEAKD CrCDKA/1-327 GVPSTAIREISFLKELRHDN----VVRLYDVLYSDR------------RLYLVFEFLDLD CrCDKB/1-325 GVPSTTLREISLLQMLSESN---HIVKLLCVEHTEEN--------NKPCLYLVFEYLNTD HsCDK1/1-297 GVPSTAIREISLLKELRHPN----IVSLQDVLMQDS------------RLYLIFEFLSMD MmCDK1/1-297 GVPSTAIREISLLKELRHPN----IVSLQDVLMQDS------------RLYLIFEFLSMD AtCDKA-1/1-294 GVPSTAIREISLLKEMQHSN----IVKLQDVVHSEK------------RLYLVFEYLDLD SpCdc2/1-297 GVPSTAIREISLLKEVNDENNRSNCVRLLDILHAES------------KLYLVFEFLDMD ScCdc28/1-298 GVPSTAIREISLLKELKDDN----IVRLYDIVHSDAH-----------KLYLVFEFLDLD OtCDKA/1-296 GVPSTAIREISLLKELRHEN----VVSLLEVIHEET------------KLYLVFEYLDLD AtCDKB2-2/1-315 GVPPTTLREISILRMLARDP---HIVRLMDVKQGINK-------EGKTVLYLVFEYVDTD AtCDKB2-1/1-313 GVPSTTLREISILRMLARDP---HVVRLMDVKQGLSK-------EGKTVLYLVFEYMDTD AtCDKB1-2/1-311 GIPPTALREISLLQMLSQSI---YIVRLLCVEHVIQSKDSTVSHSPKSNLYLVFEYLDTD OtCDKB/1-329 GVPSTALREVSLLQMLSESP---YIVRLLRVEHVEED--------GKAMLYLVFEFLDQD AtCDKB1-1/1-309 GIPPTALREISLLQMLSTSI---YVVRLLCVEHVHQP--STKSQSTKSNLYLVFEYLDTD gi|4502743|ref|NP_001790.1|/1-346 GINRTALREIKLLQELSHPN----IIGLLDAFGHKSN------------ISLVFDFMETD CrCDKA/1-327 LKKQMD--QTP-N-FSRNQRVIKMYMWQMLSGIAFCHSRRILHRDLKPQNLLIDRSRNTL CrCDKB/1-325 MKKWMDRHGKGPA-HPLPSMHIKSMVYQLIKGVAYCHMHGVLHRDLKPQNLLVDDEKMCL HsCDK1/1-297 LKKYLD--SIPPG-QYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLID-DKGTI MmCDK1/1-297 LKKYLD--SIPPG-QFMDSSLVKSYLHQILQGIVFCHSRRVLHRDLKPQNLLID-DKGTI AtCDKA-1/1-294 LKKHMD--STP-D-FSKDLHMIKTYLYQILRGIAYCHSHRVLHRDLKPQNLLIDRRTNSL SpCdc2/1-297 LKKYMDRISETGA-TSLDPRLVQKFTYQLVNGVNFCHSRRIIHRDLKPQNLLID-KEGNL ScCdc28/1-298 LKRYMEGIPKD---QPLGADIVKKFMMQLCKGIAYCHSHRILHRDLKPQNLLIN-KDGNL OtCDKA/1-296 LKKHMD--SSP-H-ISNDRMVIKGYVYQICAGIAFCHSHRVLHRDLKPQNLLIDTTNNVL AtCDKB2-2/1-315 LKKFIRSFR--QAGQNIPQNTVKCLMYQLCKGMAFCHGHGVLHRDLKPHNLLMDRKTMTL AtCDKB2-1/1-313 VKKFIRSFR--STGKNIPTQTIKSLMYQLCKGMAFCHGHGILHRDLKPHNLLMDPKTMRL AtCDKB1-2/1-311 LKKFIDSHRKGSNPRPLEASLVQRFMFQLFKGVAHCHSHGVLHRDLKPQNLLLDKDKGIL OtCDKB/1-329 LKQFMDLTGRGPS-NPLPTTTVQNYMYQLCLGCAHLHKHGVMHRDLKPQNMLVDKAKNLL AtCDKB1-1/1-309 LKKFIDSYRKGPNPKPLEPFLIQKLMFQLCKGVAHCHSHGVLHRDLKPQNLLLVKDKELL gi|4502743|ref|NP_001790.1|/1-346 LEVIIKDNS-----LVLTPSHIKAYMLMTLQGLEYLHQHWILHRDLKPNNLLLD-ENGVL CrCDKA/1-327 KLADFGLARAFGIPVRAYTHEVVTLWYRAPEILLGSKTYSTPVDIWSIGCIFAEMVNHRP CrCDKB/1-325 KVADLGLGRHFSVPLKSYTHEIVTLWYRAPEVLLGATHYATPVDMWSVGCIFAELVRKAP HsCDK1/1-297 KLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKP MmCDK1/1-297 KLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKP AtCDKA-1/1-294 KLADFGLARAFGIPVRTFTHEVVTLWYRAPEILLGSHHYSTPVDIWSVGCIFAEMISQKP SpCdc2/1-297 KLADFGLARSFGVPLRNYTHEIVTLWYRAPEVLLGSRHYSTGVDIWSVGCIFAEMIRRSP ScCdc28/1-298 KLGDFGLARAFGVPLRAYTHEIVTLWYRAPEVLLGGKQYSTGVDTWSIGCIFAEMCNRKP OtCDKA/1-296 KLADFGLARAFGIPVRAYTHEVVTLWYRAPEILLGVRTYSTPVDVWSIGCIMAEMVNHAP AtCDKB2-2/1-315 KIADLGLARAFTLPMKKYTHEILTLWYRAPEVLLGATHYSTGVDMWSVGCIFAELVTKQA AtCDKB2-1/1-313 KIADLGLARAFTLPMKKYTHEILTLWYRAPEVLLGATHYSTAVDMWSVGCIFAELVTNQA AtCDKB1-2/1-311 KIADLGLSRAFTVPLKAYTHEIVTLWYRAPEVLLGSTHYSTAVDIWSVGCIFAEMIRRQA OtCDKB/1-329 KIADLGLGRAFSVPVKSYTHEIVTLWYRAPEVLLGGSHYSTPVDMWSVGCMFAEMARKQP AtCDKB1-1/1-309 KIADLGLGRAFTVPLKSYTHEIVTLWYRAPEVLLGSTHYSTGVDMWSVGCIFAEMVRRQA gi|4502743|ref|NP_001790.1|/1-346 KLADFGLAKSFGSPNRAYTHQVVTRWYRAPELLFGARMYGVGVDMWAVGCILAELLLRVP CrCDKA/1-327 LFPGDSEIDQLHKIFQLLGTPDEAMWAGCSALPDFKDTFPKWRPQNLGAAVPTLSPEGVD CrCDKB/1-325 LFPGDSEYQQLLHIFKLLGTPSEDTWPGVTKLRDWH-EWPQWQPQDLHRIFPSLDDSGID HsCDK1/1-297 LFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLD MmCDK1/1-297 LFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLD AtCDKA-1/1-294 LFPGDSEIDQLFKIFRIMGTPYEDTWRGVTSLPDYKSAFPKWKPTDLETFVPNLDPDGVD SpCdc2/1-297 LFPGDSEIDEIFKIFQVLGTPNEEVWPGVTLLQDYKSTFPRWKRMDLHKVVPNGEEDAIE ScCdc28/1-298 IFSGDSEIDQIFKIFRVLGTPNEAIWPDIVYLPDFKPSFPQWRRKDLSQVVPSLDPRGID OtCDKA/1-296 LFPGDSEIDELFKIFKTLGTPGETLWPEAKQLPDYQEGFPKWKAKPWESLCPALDEAGVD AtCDKB2-2/1-315 IFAGDSELQQLLRIFRLLGTPNEEVWPGVSKLKDWH-EYPQWKPLSLSTAVPNLDEAGLD AtCDKB2-1/1-313 IFQGDSELQQLLHIFKLFGTPNEEMWPGVSTLKNWH-EYPQWKPSTLSSAVPNLDEAGVD AtCDKB1-2/1-311 LFPGDSEFQQLLHIFRLLGTPTEQQWPGVMALRDWH-VYPKWEPQDLSRAVPSLSPEGID OtCDKB/1-329 LFPGDSELQQLLHIFKLLGTPSEQVWPGVSNLRDWH-EFPQWKPQDLAKLIPQLDAHGID AtCDKB1-1/1-309 LFPGDSEFQQLLHIFRLLGTPTEQQWPGVSTLRDWH-VYPKWEPQDLTLAVPSLSPQGVD gi|4502743|ref|NP_001790.1|/1-346 FLPGDSDLDQLTRIFETLGTPTEEQWPDMCSLPDYV-TFKSFPGIPLHHIFSAAGDDLLD CrCDKA/1-327 LLARMLVYTPQHRITASAAMEHAYFNEIRDTMRAAVAPGGGGMGGGMGGMGAMGGMGGGM CrCDKB/1-325 LMKRCFAYDPAIRISAKEAINHPYFDDL-DKVTVDALESQEVRDRMAEAAAANVVC---- HsCDK1/1-297 LLSKMLIYDPAKRISGKMALNHPYFNDLD-----------NQIKKM-------------- MmCDK1/1-297 LLSKMLVYDPAKRISGKMALKHPYFDDLD-----------NQIKKM-------------- AtCDKA-1/1-294 LLSKMLLMDPTKRINARAALEHEYFKDL------------GGMP---------------- SpCdc2/1-297 LLSAMLVYDPAHRISAKRALQQNYLRDFH------------------------------- ScCdc28/1-298 LLDKLLAYDPINRISARRAAIHPYFQES-------------------------------- OtCDKA/1-296 LLRSLLQYSPEKRISAKYATQHRWFDDYR-----------EHIGR--------------- AtCDKB2-2/1-315 LLSKMLEYEPAKRISAKKAMEHPYFDDLPDKSSL-------------------------- AtCDKB2-1/1-313 LLSKMLQYEPAKRISAKMAMEHPYFDDLPEKSSL-------------------------- AtCDKB1-2/1-311 LLTQMLKYNPAERISAKAALDHPYFDSL-DKSQF-------------------------- OtCDKB/1-329 LLQKLLVFDPAKRIHATDALEHPYFDSL-DKTQFEKIEREFEIEFLREKENRMAQ----- AtCDKB1-1/1-309 LLTKMLKYNPAERISAKTALDHPYFDSL-DKSQF-------------------------- gi|4502743|ref|NP_001790.1|/1-346 LIQGLFLFNPCARITATQALKMKYFSNRPGPTPGCQLPRPNCPVETLKEQSNPALAIKRK CrCDKA/1-327 GVMR------------ CrCDKB/1-325 ---------------- HsCDK1/1-297 ---------------- MmCDK1/1-297 ---------------- AtCDKA-1/1-294 ---------------- SpCdc2/1-297 ---------------- ScCdc28/1-298 ---------------- OtCDKA/1-296 ---------------- AtCDKB2-2/1-315 ---------------- AtCDKB2-1/1-313 ---------------- AtCDKB1-2/1-311 ---------------- OtCDKB/1-329 ---------------- AtCDKB1-1/1-309 ---------------- gi|4502743|ref|NP_001790.1|/1-346 RTEALEQGGLPKKLIF