WblA_ch, a Pivotal Activator of Natamycin Biosynthesis and Morphological Differentiation in Streptomyces chattanoogensis L10, Is Positively Regulated by AdpA_ch

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  Diagram showing wblA_ch disruption via double crossover, confirmation of constructed wblA_ch mutant by PCR amplification, and Southern blot analysis of wild-type and ΔwblA_ch mutant strains (Fig. S1); effect of wblA_ch disruption on morphological differentiation (Fig. S2); HPLC analysis of culture filtrates from the wild-type strain and the wblA_ch overexpression strain (Fig. S3); EMSA analysis of the AdpA_ch binding sequences and mutated sequences (Fig. S4); peak area of natamycin and yellow pigment, determined by HPLC analysis, from the WT strain and wblA_ch overexpression strain (Table S1).

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