Structural Insights into 2,2′-Azino-Bis(3-Ethylbenzothiazoline-6-Sulfonic Acid) (ABTS)-Mediated Degradation of Reactive Blue 21 by Engineered Cyathus bulleri Laccase and Characterization of Degradation Products

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  Strategy for mutagenesis of the 816-nt segment of the C. bulleri lcc⁺ gene (Fig. S1); oxidation potential of RB21 and comparison of redox potentials of WtLcc and the mutant variants Lcc-35, Lcc-61, and Lcc-62 (Fig. S2); relative ABTS-oxidizing activities of C-terminus laccase mutants (Fig. S3); sequence alignment of the 816-nt segment (towards the C terminus) of the wild-type lcc⁺ gene of C. bulleri with bacterial, fungal, and plant laccases, using Clustal Omega (Fig. S4).

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