Homeostasis of N-α-Terminal Acetylation of EsxA Correlates with Virulence in Mycobacterium marinum

Supplemental material

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• Supplemental file 1 -

  Fig. S1. The 120A3 strain does not have a growth defect in vitro. Fig. S2. The 120A3 strain is attenuated for growth in RAW246.7 cells. Fig. S3. MRM transitions used in this study. Fig. S4. Raw data for absolute quantification of EsxA using heavy-isotope dilution. Fig. S5. MMAR_0039 is required for the export of several Esx-1 substrates. Fig. S6. Spectra demonstrating N-α-terminal acetylation of EspB and EspF. Fig. S7. Analysis of N-α-terminal Ac EspF and EsxN peptides in the CL, CF, and SP.

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• Supplemental file 2 -

  Table S1. Strains, plasmids, and oligonucleotides used in this study.

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