Scalable and Versatile Genome Editing Using Linear DNAs with Microhomology to Cas9 Sites in Caenorhabditis elegans

Files in this data supplement:

  • Supporting Information: Figures S1-S3, Tables S1-S5, and File S1 (PDF, 878 KB)
  • Figure S1: Schematic representation of deletion/insertion experiments on K08F4.2 gene. (PDF, 223 KB)
  • Figure S2: PCR and restriction digest analysis of F1 pools from Exp. 1. (PDF, 403 KB)
  • Figure S3: Whole genome sequencing of strains obtained in Exp. 1 and 12. (PDF, 429 KB)
  • Table S1: Expanded version of Table 1. (.xlsx, 501 KB)
  • Table S2: sgRNAs sequences. (PDF, 120 KB)
  • Table S3: Repair template sequences. (PDF, 147 KB)
  • Table S4: GFP edit frequencies by brood and day of egg laying. (PDF, 81 KB)
  • Table S5: Strain list. (PDF, 120 KB)
  • File S1: Protocol: Seamless editing of the C. elegans genome using CRISPR/Cas9. (PDF, 185 KB)