Supplementary Figure2. Alignment ofthe transcripts produced from the mutant TCIRG1 tothe genomic sequence. Exons aredepicted in black, introns in blue. WT transcript GCCTGGCTGCCAACCACTTGAGCTTCCTCAACTCCTTCAAGATGAAGATGTCCGTCATCCTGGGCGTCGTG Aberranttranscript1 GCCTGGCTGCCAACCACTTGAGCTTCCTCAACTCCTTCAAGATGAAGATGTCCGTCATCCTGGGCGTCGTG Aberranttranscript2 GCCTGGCTGCCAACCACTTGAGCTTCCTCAACTCCTTCAAGATGAAGATGTCCGTCATCCTGGGCGTCGTG Aberranttranscript3 GCCTGGCTGCCAACCACTTGAGCTTCCTCAACTCCTTCAAGATGAAGATGTCCGTCATCCTGGGCGTCGTG Aberranttranscript4 GCCTGGCTGCCAACCACTTGAGCTTCCTCAACTCCTTCAAGATGAAGATGTCCGTCATCCTGGGCGTCGT--- TCIRG1 gene TGGAGCCTGGCTGCCAACCACTTGAGCTTCCTCAACTCCTTCAAGATGAAGATGTCCGTCATCCTGGGCGTCGTG exon 14 WT transcript CACATGGCCTTTGGGGTGGTCCTCGGAGTCTTCAACCACGT---------------------------------------------------------------------- Aberranttranscript1 CACATGGCCTTTGGGGTGGTCCTCGGAGTCTTCAACCACGT---------------------------------------------------------------------- Aberranttranscript2 CACATGGCCTTTGGGGTGGTCCTCGGAGTCTTCAACCACGT---------------------------------------------------------------------- Aberranttranscript3 CACATGGCCTTTGGGGTGGTCCTCGGAGTCTTCAACCACGT---------------------------------------------------------------------- Aberranttranscript4 --------------------------------------------------------------------------------------------------------------------------------------------------------- TCIRG1 gene CACATGGCCTTTGGGGTGGTCCTCGGAGTCTTCAACCACGTGTGAGGGCCAAGGCTGCCCGGGGGACGGGAGGC exon 14 intron 14 WT transcript -------------------------------------------------------------------------------------------------------GCACTTTGGCCAGAGGCACCGGCT Aberranttranscript1 -------------------------------------------------------------------------------------------------------GCACTTTGGCCAGAGGCACCGGCT Aberranttranscript2 -------------------------------------------------------------------------------------------------------GCACTTTGGCCAGAGGCACCGGCT Aberranttranscript3 -------------------------------------------------------------------------------------------------------GCACTTTGGCCAGAGGCACCGGCT Aberranttranscript4 -------------------------------------------------------------------------------------------------------GCACTTTGGCCAGAGGCACCGGCT TCIRG1 gene TGGCAGGCCAGAGTGGGCCCCAGTGAGCACACCTCCCTCTTGCCCGCCAGGCACTTTGGCCAGAGGCACCGGCT intron 14 exon 15 WT transcript GCTGCTGGAGACGCTGCCGGGCTCACCTTCCTGCTGGGACTCTTCGGTTACCTCGATGTTCCTAGTCATCTACAAG Aberranttranscript1 GCTGCTGGAGACGCTGCCGGGCTCACCTTCCTGCTGGGACTCTTCGGTTACCTCGATGTTCCTAGTCATCTACAAG Aberranttranscript2 GCTGCTGGAGACGCTGCCGGGCTCACCTTCCTGCTGGGACTCTTCGGTTACCTCGATGTTCCTAGTCATCTACAAG Aberranttranscript3 GCTGCTGGAGACGCTGCCGGGCTCACCTTCCTGCTGGGACTCTTCGGTTACCTCGATGTTCCTAGTCATCTACAAG Aberranttranscript4 GCTGCTGGAGACGCTGCCGGGCTCACCTTCCTGCTGGGACTCTTCGGTTACCTCGATGTTCCTAGTCATCTACAAG TCIRG1 gene GCTGCTGGAGACGCTGCCGGAGCTCACCTTCCTGCTGGGACTCTTCGGTTACCTCGTGTTCCTAGTCATCTACAAG exon 15 WT transcript TGGCTGTGTGTCTGGGCTGCCAGGGCCGCCTCGGCCCCCAGCATCCTCATCCACTTCATCAACATGTTCCTCTTCT Aberranttranscript1 TGGCTGTGTGTCTGGGCTGCCAGGGCCGCCTCGGCCCCCAGCATCCTCATCCACTTCATCAACATGTTCCTCTTCT Aberranttranscript2 TGGCTGTGTGTCTGGGCTGCCAGGGCCGCCTCGGCCCCCAGCATCCTCATCCACTTCATCAACATGTTCCTCTTCT Aberranttranscript3 TGGCTGTGTGTCTGGGCTGCCAGGGCCGCCTCGGCCCCCAGCATCCTCATCCACTTCATCAACATGTTCCTCTTCT Aberranttranscript4 TGGCTGTGTGTCTGGGCTGCCAGGGCCGCCTCGGCCCCCAGCATCCTCATCCACTTCATCAACATGTTCCTCTTCT TCIRG1 gene TGGCTGTGTGTCTGGGCTGCCAGGGCCGCCTCGGCCCCCAGCATCCTCATCCACTTCATCAACATGTTCCTCTTCT exon 15 WT transcript CCCACAGCCCCAGCAACAGGCTGCTCTACCCCCGGCAG---------------------------------------------------------------------------- Aberranttranscript1 CCCACAGCCCCAGCAACAGGCTGCTCTACCCCCG------------------------------------------------------------------------------------ Aberranttranscript2 CCCACAGCCCCAGCAACAGGCTGCTCTACCCCCG------------------------------------------------------------------------------------ Aberranttranscript3 CCCACAGCCCCAGCAACAGGCTGCTCTACCCCCGGCAGGTGGGCTGCGGCTGGTGGGGGCCGGGCTCACACGGC Aberranttranscript4 CCCACAGCCCCAGCAACAGGCTGCTCTACCCCCGGCAG---------------------------------------------------------------------------- TCIRG1 gene CCCACAGCCCCAGCAACAGGCTGCTCTACCCCCGGCAGGTGGGCTGCGGCTGGTGGGGGCCGGGCTCACACGGC exon 15 intron 15 WT transcript ---------------------------------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript1 ---------------------------------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript2 ---------------------------------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript3 CTCATGGGGACCCCGCGGTCACAGGGCCACTGGGAGCTGCAAGATCCTCGTCCGAGAAACGGGGATGCAGGCCC Aberranttranscript4 ---------------------------------------------------------------------------------------------------------------------------------------------------------- TCIRG1 gene CTCATGGGGACCCCGCGGTCACAGGGCCACTGGGAGCTGCAAGATCCTCGTCCGAGAAACGGGGATGCAGGCCC intron 15 WT transcript ---------------------------------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript1 ---------------------------------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript2 ---------------------------------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript3 CGGGCCGTGCAGACCAGGGCCGTCAGAG------------------------------------------------------------------------------------------------ Aberranttranscript4 ---------------------------------------------------------------------------------------------------------------------------------------------------------- TCIRG1 gene CGGGCCGTGCAGACCAGGGCCGTCAGAGGTGATGGTGTGCATCTTTAGCAGGTGGCACAACTGGCACTGGGAAC intron 15 WT transcript ---------------------------------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript1 ---------------------------------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript2 ---------------------------------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript3 ---------------------------------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript4 ---------------------------------------------------------------------------------------------------------------------------------------------------------- TCIRG1 gene CGGGGGTCCCTTCCCTCAGGAGTCCTTATGGAGGCAGACCCTCACCCAGTGAGGGCACGGAGCCCCCGGGGGCC intron 15 WT transcript ---------------------------------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript1 ---------------------------------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript2 ---------------------------------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript3 ---------------------------------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript4 ---------------------------------------------------------------------------------------------------------------------------------------------------------- TCIRG1 gene CTGGAGGGAGGAGCGACCGCAGGCTCTCTGGGCCCCGTGACTGCTGTGACTCAGGTTCCTTTGCAGGTGTGCACA intron 15 WT transcript --------------------------------------------------------------------------GAGGTGGTCCAGGCCACGCTGGTGGTCCTGGCCTTGGCC Aberranttranscript1 ---------------------------------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript2 --------------------------------------------------------------------------GAGGTGGTCCAGGCCACGCTGGTGGTCCTGGCCTTGGCC Aberranttranscript3 --------------------------------------------------------------------------GAGGTGGTCCAGGCCACGCTGGTGGTCCTGGCCTTGGCC Aberranttranscript4 --------------------------------------------------------------------------GAGGTGGTCCAGGCCACGCTGGTGGTCCTGGCCTTGGCC TCIRG1 gene GCAGGGACGCCCTGACTCTCGCCCTCTCCCTGGCAGGAGGTGGTCCAGGCCACGCTGGTGGTCCTGGCCTTGGCC intron 15 exon 16 WT transcript ATGGTGCCCATCCTGCTGCTTGGCACACCCCTGCACCTGCTGCACCGCCACCGCCGCCGCCTGCGGAGGAGGCCC Aberranttranscript1 ---------------------------------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript2 ATGGTGCCCATCCTGCTGCTTGGCACACCCCTGCACCTGCTGCACCGCCACCGCCGCCGCCTGCGGAGGAGGCCC Aberranttranscript3 ATGGTGCCCATCCTGCTGCTTGGCACACCCCTGCACCTGCTGCACCGCCACCGCCGCCGCCTGCGGAGGAGGCCC Aberranttranscript4 ATGGTGCCCATCCTGCTGCTTGGCACACCCCTGCACCTGCTGCACCGCCACCGCCGCCGCCTGCGGAGGAGGCCC TCIRG1 gene ATGGTGCCCATCCTGCTGCTTGGCACACCCCTGCACCTGCTGCACCGCCACCGCCGCCGCCTGCGGAGGAGGCCC exon 16 WT transcript GCTGACCGACAG--------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript1 ---------------------------------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript2 GCTGACCGACAG--------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript3 GCTGACCGACAG--------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript4 GCTGACCGACAG--------------------------------------------------------------------------------------------------------------------------------- TCIRG1 gene GCTGACCGACAGGTGGGACCGGGGCCTAAGGTGTGGGGGGCTGCTTGCGGAGAGGCCACTGTCCGGTGTGTCCC exon 16 intron 16 WT transcript ---------------------------------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript1 ---------------------------------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript2 ---------------------------------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript3 ---------------------------------------------------------------------------------------------------------------------------------------------------------- Aberranttranscript4 ---------------------------------------------------------------------------------------------------------------------------------------------------------- TCIRG1 gene TGACTCCTCGCTTCCTGACAGACTCCTGAGTGGCCAGGAGCAGGCCTGGCGGGTGGTGGGGGACCTCCTGGGGCT intron 16 WT transcript ----------------------------------------------------------------------GAGGAAAACAAGGCCGGGTTGCTGGACCTGCCTGACGCAT Aberranttranscript1 ----------------------------------------------------------------------GAGGAAAACAAGGCCGGGTTGCTGGACCTGCCTGACGCAT Aberranttranscript2 ----------------------------------------------------------------------GAGGAAAACAAGGCCGGGTTGCTGGACCTGCCTGACGCAT Aberranttranscript3 ----------------------------------------------------------------------GAGGAAAACAAGGCCGGGTTGCTGGACCTGCCTGACGCAT Aberranttranscript4 ----------------------------------------------------------------------GAGGAAAACAAGGCCGGGTTGCTGGACCTGCCTGACGCAT TCIRG1 gene GGAGTGCTGCCAACACTGCCTGCTCATGCCCCAGGAGGAAAACAAGGCCGGGTTGCTGGACCTGCCTGACGCAT intron 16 exon 17 WT transcript CTGTGAATGGCTGGAGCTCC--------------- Aberranttranscript1 CTGTGAATGGCTGGAGCTCC--------------- Aberranttranscript2 CTGTGAATGGCTGGAGCTCC--------------- Aberranttranscript3 CTGTGAATGGCTGGAGCTCC--------------- Aberranttranscript4 CTGTGAATGGCTGGAGCTCC--------------- TCIRG1 gene CTGTGAATGGCTGGAGCTCCGATGA….. exon 17