Supplementary Figure 1
Supplementary Figure 1. Induction profiles of various known DNA sensors and adaptors in the glioma microenvoironment.
Total RNA from glioma-bearing brain samples were analyzed for the indicated mRNA expression by qRT-PCR as in Figure 1. The primers used for specific mRNA detection are: Ifi16 forward: GACAACCAAGAGCAATACACCA, reverse: ATCAGTTTGCCCAATCCAGAAT; STING forward: ACCTAGCCTCGCACGAACT, reverse: CCAACTGAGGTATATGTCAGCAG; and Aim2 forward: GTCCTCAAGCTAAGCCTCAGA, reverse CACCGTGACAACAAGTGGAT.

Supplementary Figure 2

Supplementary Figure 2. STING agonist promotes type-I IFN signaling in BILs.
tdTomato mice bearing GL261-luc gliomas received intratumoral injection of control solvent or c-di-GMP. On day 5 following the treatment, CD11c+, CD8+ T, and CD4+ T BILs were evaluated for GFP expression. Representative flow data are shown. Cumulative data on the percentage of GFP+ cells in each leukocyte population in the right panel (3 mice/group). *p < 0.05 based on Student’s t-test.