A holin and an endopeptidase are essential for chitinolytic protein secretion in Serratia marcescens

Files in this Data Supplement:

• Supplemental Material (PDF)
• Video 1 -
  Fate of strain FTG005 GFP-positive cells upon time-lapse imaging—dividing cell. Strain FTG005 (ϕchiA::gfp) was grown for 18 h in minimal media before being diluted and applied to a microscope slide. Images were analyzed by time-lapse epifluorescence microscopy using a wide-field microscope (DeltaVision Core; Applied Precision) mounted on an inverted stand (IX71; Olympus) with a 60×, 1.4 NA lens (Olympus) and a camera (CoolSNAP HQ; Photometrics) with DIC and fluorescence optics. The left-hand image is the merge of the DIC and FITC channel (false-colored green), the center image is the DIC channel, and the right-hand image is the FITC channel (shown in white). Frames were taken every 15 min for 4 h and 30 min and are at four frames per second for display.
• Video 2 -
  Fate of strain FTG005 GFP-positive cells upon time-lapse imaging—quiescent cell. Strain FTG005 (ϕchiA::gfp) was grown for 18 h in minimal media before being diluted and applied to a microscope slide. Images were analyzed by time-lapse epifluorescence microscopy using a wide-field microscope (DeltaVision Core; Applied Precision) mounted on an inverted stand (IX71; Olympus) with a 60×, 1.4 NA lens (Olympus) and a camera (CoolSNAP HQ; Photometrics) with DIC and fluorescence optics. The left-hand image is the merge of the DIC and FITC channel (false-colored green), the center image is the DIC channel, and the right-hand image is the FITC channel (shown in white). Frames were taken every 15 min for 4 h and 30 min and are at four frames per second for display.