TableS1.  Detailed description of thelines used in this study 
 
Species 
 
Line
 
Location 
 
Elevation(m) 
 
Statusa 
 
Sequencingb 
Position in 
thetreec 
D. yakuba 12 Y Abdjan, 
Ivory Coast
 
- no-HZ ABI no-HZC
D. yakuba 21 Y Abdjan, 
Ivory Coast
 
- no-HZ ABI no-HZC
D. yakuba 42 Y Abdjan, 
Ivory Coast
 
- no-HZ ABI no-HZC
D. yakuba ST Taïforest, 
Ivory Coast
 
- no-HZ ABI no-HZC
D. yakuba CAM Kounden 
Plateau, 
Cameroon 
- no-HZ ABI no-HZC
D. yakuba GN46 Gabon 
 
 
- no-HZ ABI no-HZC
D. yakuba GN110 Gabon 
 
 
- no-HZ ABI HZC
D. yakuba ZW002A Zimbabwe
 
 
- no-HZ ABI no-HZC
D. yakuba ZW121 Zimbabwe
 
 
- no-HZ ABI no-HZC
D. yakuba ZW162 Zimbabwe
 
 
- no-HZ ABI no-HZC
D. yakuba Ky221 Kenya
 
 
- no-HZ ABI no-HZC
D. yakuba TZ032 Tanzania 
 
 
- no-HZ ABI HZC
D. yakuba TZ025 Tanzania 
 
 
- no-HZ ABI no-HZC
D. yakuba LP6 São Tomé Sealevel no-HZ ILMN no-HZCCity, 
São Tomé
D. yakuba SN2 São 
Nicolau, 
São Tomé
- no-HZ ABI HZC
D. yakuba BS3 Bom
Sucesso, 
São Tomé
1,090 HZ ILMN no-HZC
D. yakuba BS4 Bom
Sucesso, 
São Tomé
1,090 HZ ILMN HZC
D. yakuba Field1 Obo 
Reserve, 
São Tomé
1,090-1,190 HZ ILMN HZC
D. yakuba Field2 Obo 
Reserve, 
São Tomé
1,090-1,190 HZ ILMN no-HZC
D. yakuba Field3 Obo 
Reserve, 
São Tomé
1,090-1,190 HZ ILMN HZC
D. yakuba BOSSU1153.1 Obo 
Reserve, 
São Tomé
1,153 HZ ILMN no-HZC
D. yakuba OBAT1200.5 Obo 
Reserve, 
São Tomé
1,200 HZ ILMN HZC
D. yakuba COST1235.2 Obo 
Reserve, 
São Tomé
1,235 HZ ILMN HZC
D. yakuba Rain1 Obo 
Reserve, 
São Tomé
1,191-1,390 HZ ILMN HZC
D. yakuba Rain2 Obo 
Reserve, 
São Tomé
1,191-1,390 HZ ILMN HZC
D. yakuba Rain3 Obo 
Reserve, 
São Tomé
1,191-1,390 HZ ILMN HZC
D. yakuba Rain4 Obo 
Reserve, 
São Tomé
1,191-1,390 HZ ILMN no-HZC
D. yakuba Rain5 Obo 
Reserve, 
São Tomé
1,191-1,390 HZ ILMN HZC
D. yakuba Rain6 Obo 
Reserve, 
1,191-1,390 HZ ILMN HZCSão Tomé
D. santomea BS11 Bom
Sucesso, 
São Tomé
1,090 HZ ILMN HZC
D. santomea BS13 Bom
Sucesso, 
São Tomé
1,090 HZ ILMN no-HZC
D. santomea Field4 Obo 
Reserve, 
São Tomé
1,090-1,190 HZ ILMN no-HZC
D. santomea Field6 Obo 
Reserve, 
São Tomé
1,090-1,190 HZ ILMN no-HZC
D. santomea COST1235.1 Obo 
Reserve, 
São Tomé
1,235 HZ ILMN HZC
D. santomea STO.4 Obo 
Reserve, 
São Tomé
1,150-1,350 HZ ILMN HZC
D. santomea STO.7 Obo 
Reserve, 
São Tomé
1,150-1,350 HZ ILMN HZC
D. santomea STO.18 Obo 
Reserve, 
São Tomé
1,150-1,350 HZ ILMN HZC
D. santomea Rain36 Obo 
Reserve, 
São Tomé
1,191-1,390 HZ ILMN HZC
D. santomea Rain40 Obo 
Reserve, 
São Tomé
1,191-1,390 HZ ILMN no-HZC
D. santomea Thena2 Obo 
Reserve, 
São Tomé
1,391-1,415 HZ ILMN no-HZC
D. santomea Thena5 Obo 
Reserve, 
São Tomé
1,391-1,415 HZ ILMN no-HZC
D. santomea CAR1600.3 Pico 
Calvario, 
São Tomé
1,600 no-HZ ILMN HZC
D. santomea QUIJA650.1 Rio Queijo, 
São Tomé
 
650 no-HZ ILMN no-HZC
D. santomea QUIJA650.12 Rio Queijo, 
São Tomé
 
650 no-HZ ILMN no-HZCD. santomea QUIJA650.24 Rio Queijo, 
São Tomé
  
650 no-HZ ILMN no-HZC
D. santomea Cabumbé Pico 
Cabumbé, 
São Tomé
1,050 no-HZ ILMN HZC
a HZ, linederived fromafemalecollected in theclassichybrid zoneformed by D. yakuba and 
D. santomea;no-HZ, linederived fromafemalecollected outsidetheclassichybrid zone
formed by D. yakuba and D. santomea
b ABI, Standard cyclesequencing run in an ABI 3730 GenomeAnalyzer;ILMN, Illumina
GAIIsequencing.  
c HZC, hybrid zoneclade;no-HZC, outsidethehybrid zoneclade.