Fast and Efficient Drosophila melanogaster Gene Knock-Ins Using MiMIC Transposons

Supporting Information for Vilain et al., 2014

Files in this Data Supplement:

• Supporting Information - Files S1-S2 and Figures S1-S5 (PDF, 1 MB)
• File S1 - Supporting Materials and Methods (PDF, 114 KB)
• File S2 - Bioinformatics protocol to generate Figure 4. (PDF, 255 KB)
• Figure S1 - Overview of the crossing scheme used to create double strand breaks using restriction endonucleases I-SceI and I-CreI. (PDF, 345 KB)
• Figure S2 - LRRK^HA recapitulates wild type LRRK function. (PDF, 431 KB)
• Figure S3 - Strategy for sky targeting and molecular confirmation of targeted sky and tau loci. (PDF, 439 KB)
• Figure S4 - Crossing scheme for direct targeting of a gene of interest (here on the 2nd chromosome) using a MiMIC transposon. (PDF, 336 KB)
• Figure S5 - Crossing scheme for gene targeting using CRISPR/Cas9. (PDF, 258 KB)