Files in this Data Supplement:
Supplemental text (characterization of the evolution process and the endpoint strains, analysis of mutations identified in the evolved strains, analysis of reproducibility for key mutations which enable increased fitness phenotypes, integrated genome-scale modeling); regions for each of the populations where fitness increased significantly (Table S1); regions for each of the populations where fitness increased significantly for the validation ALE (Table S2); plots of the identified jump regions and mutations found in clones isolated at various points along the ALE experiments (Fig. S1); relationship between the populations and the clones isolated from the populations are weakly correlated (Fig. S2); pairwise comparisons of all phenotypic data were made for each endpoint isolate (Fig. S3); validation ALE jumps and sequences (Fig. S4); Δ82-bp deletion in pyrE/rph penetration by PCR (Fig. S5); transcriptomic data: enriched differentially expressed protein-coding genes from evolved strains (Fig. S6).
PDF, 788K
Growth rate, flask, generations, and cumulative cell divisions (CCD) for each experiment (Data Set S1).
XLSX, 383K
Mutations detected for each of the isolated clones across the different experiments (Data Set S2).
XLSX, 82K
Transcriptomic analysis: expression level and differentially expressed genes for each strain (Data Set S3).
XLSX, 1.2M