Supplemental Data
Files in this Data Supplement:
- Supplementary Video 1 (.mov, 3.4 MB) - SPT of FLAG-LPA2 in NIH 3T3-LPA2 cell in the absence or presence of LPA.
- Supplementary video 2 (.mov, 4.9 MB) - Calcium mobilization in Fluo 4 labeled DKO-LPA2 MEFs in the presence of a gradient of LPA generated by a micropipette. White arrows indicate the site of micropipette.
- Supplementary video 3 (.mov, 2.4 MB) - Ca2+ puffs are triggered by an LPA gradient in DKO-LPA2 MEFs. Cells were exposed to a gradient generated by a micropipette containing 200 nM LPA. White arrows represent the site of micropipette.
- Supplementary video 4 (.mov, 2.8 MB) - Chemotaxis of DKO-LPA2 MEFs. Cells were starved in serum-free medium for 6 hours and then exposed to a gradient produced by a micropipette containing 200 nM LPA.
- Supplementary video 5 (.mov, 1.3 MB) - Nondirectional migration of DKO-LPA2 MEFs. Cells were starved in serum-free medium for 6 hour and then exposed to 200nM uniform LPA.