Autotransporter-Based Antigen Display in Bacterial Ghosts

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    Sequences of the φX174 lysE and E. coli asd genes and primers used in this study (Table S1), DNA localization as a marker for ghost formation (Fig. S1), growth- and lysis protein E-induced lysis of E. coli MC4100Δasd-based cultures (Fig. S2), effect of omitting DAP from the culture medium on the integrity of E. coli MC4100Δasd-derived ghosts and cells (Fig. S3), flow cytometry-based analysis of E. coli bacterial ghosts displaying HbpD-ESAT6 (Fig. S4), flow cytometry-based analysis of Salmonella ghost morphology (Fig. S5), and flow cytometry-based analysis of Salmonella bacterial ghosts displaying HbpD-ESAT6 (Fig. S6).

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