Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie07 - Supplementary Video S7: Ventral enclosure for a WASP null embryo. Spinning disc confocal microscopy of epidermal cells expressing Lifeact-GFP filmed over 60 minutes. Movie accelerated 180x. Still images are shown in Supplementary Figure S3. Bar 15 μm.
- Movie09 - Supplementary Video S9: Ventral enclosure for an embryo expressing ΔTET-VASP (green) and Lifeact-mCherry (red) in epidermal cells, filmed over 60 minutes. Spinning disc confocal microscopy. Movie accelerated 180x. Bar 15 μm.
- Movie03 - Supplementary Video S3: Ventral enclosure for an embryo carrying reintroduced (extrachromosomal) wild-type WAVE. Spinning disc confocal microscopy of epidermal cells expressing Lifeact-GFP filmed over about 20 minutes. Movie accelerated 180x. Still images are shown in Figure 3. Bar 15 μm.
- Movie08 - Supplementary Video S8: Ventral enclosure for an embryo expressing ΔFAB-VASP (green) and Lifeact-mCherry (red) in epidermal cells, filmed over 35 minutes. Spinning disc confocal microscopy. Movie accelerated 180x. Bar 15 μm.
- Movie01 - Supplementary Video S1: Ventral enclosure for a wild type embryo. Spinning disc confocal microscopy of epidermal cells expressing Lifeact-GFP filmed over 50 minutes. Movie accelerated 180x. Still images are shown in Figure 2. Bar 15 μm.
- Movie06 - Supplementary Video S6: Ventral enclosure for an embryo expressing ΔEVH1-VASP-GFP and Lifeact-mCherry in epidermal cells, red channel shown only, filmed over about 20 minutes. Spinning disc confocal microscopy. Movie accelerated 180x. Still images are shown in Figure 3. Bar 15 μm.
- Movie04 - Supplementary Video S4: Ventral enclosure for an embryo carrying ΔPRD-WAVE. Spinning disc confocal microscopy of epidermal cells expressing Lifeact-GFP filmed over about 13 minutes. Movie accelerated 180x. Still images are shown in Figure 3. Bar 15 μm.
- Movie05 - Supplementary Video S5: Ventral enclosure for an embryo expressing wild-type VASPGFP (green) and Lifeact-mCherry (red) in epidermal cells, filmed over 51 minutes. Spinning disc confocal microscopy. Movie accelerated 180x. Bar 15 μm.
- Movie02 - Supplementary Video S2: Ventral enclosure for a VASP null embryo. Spinning disc confocal microscopy of epidermal cells expressing Lifeact-GFP filmed over 50 minutes. Movie accelerated 180x. Still images are shown in Figure 2. Bar 15 μm.