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Fig. 4. Parameters of the two-step model (Eqs. 1-4) as a function of ligand concentration were calculated by fitting TCR–MHCp-binding curves at 25°C (o) and 4°C (*). The rate equations were solved numerically and the optimization was carried out by using the GLSA program.

Fig. 5. Parameters of the two-species model (Eqs. 7-11) as a function of ligand concentration were calculated by fitting TCR–MHCp-binding curves at 25°C (o) and 4°C (*). The rate equations were solved numerically and the optimization was carried out by using the GLSA program.