Table 3. Parameters derived from analysis of the sT1 TCR–H-2Kd–PbCS(ABA) association and dissociation time courses monitored at 25° C (A) and 4° C (B) by a two-step model (Eq. 1)
A
|
[TCR], m M |
1.3 |
2.5 |
5 |
10 |
20 |
|
k12, 104 M-1•s-1 |
0.6 ± 0.1 |
0.6 ± 0.1 |
0.6 ± 0.1 |
0.6 ± 0.1 |
0.6 ± 0.1 |
|
k21, s-1 |
0.20 ± 0.03 |
0.20 ± 0.03 |
0.15 ± 0.03 |
0.17 ± 0.03 |
0.15 ± 0.03 |
|
k23, 10-3 s-1 |
5.3 ± 0.5 |
4.7 ± 0.5 |
3.3 ± 0.3 |
2.6 ± 0.3 |
1.4 ± 0.1 |
|
k32, 10-2 s-1 |
1.2 ± 0.2 |
1.2 ± 0.2 |
1.1 ± 0.2 |
1.2 ± 0.2 |
0.8 ± 0.2 |
B
|
[TCR], m M |
1.0 |
1.7 |
2.6 |
8 |
16 |
|
k12, 102 M-1•s-1 |
9.0 ± 0.3 |
9.0 ± 0.3 |
9.0 ± 0.3 |
9.1 ± 0.3 |
9.3 ± 0.3 |
|
k21, 10-2 s-1 |
7.8 ± 0.5 |
7.3 ± 0.5 |
10.8 ± 0.5 |
11.3 ± 0.5 |
8.6 ± 0.5 |
|
k23, 10-2 s-1 |
2.6 ± 0.3 |
2.0 ± 0.3 |
3.2 ± 1.0 |
2.6 ± 0.3 |
2.2 ± 0.3 |
|
k32, 10-2 s-1 |
1.0 ± 0.2 |
1.2 ± 0.2 |
0.8 ± 0.2 |
0.7 ± 0.2 |
0.7 ± 0.2 |