Discovery of Supernumerary B Chromosomes in Drosophila melanogaster

Files in this data supplement:

  • Supporting Information: Figures S1-S8, Files S1-S4, and Table S1 (PDF, 574 KB)
  • Figure S1: B chromosomes are composed of heterochromatin. (PDF, 162 KB)
  • Figure S2: B chromosomes do not contain heterochromatic sequences from either the 2nd or the 3rd chromosomes. (PDF, 149 KB)
  • Figure S3: B chromosomes appear to contain 2 CID foci in an oocyte from a mtrm126/TM3 female. (PDF, 206 KB)
  • Figure S4: Crosses to introduce B chromosomes into a wild-type stock. (PDF, 238 KB)
  • Figure S5: Simulation of 1,000 generations of sibling crosses. (PDF, 217 KB)
  • Figure S6: Simulation of 10 generations of outcrossing. (PDF, 137 KB)
  • Figure S7: Some PEV reporters were unaffected by the presence of B chromosomes. (PDF, 139 KB)
  • Figure S8: Other mtrm mutant stocks, including the progenitor of the mtrm126 stock, do not contain B chromosomes. (PDF, 162 KB)
  • File S1: Multiple B chromosomes are observed in a living oocyte from a mtrm126/TM3 mother. (.avi, 1 MB)
  • File S2: Multiple B chromosomes are observed in a living oocyte from a mtrm126/TM3 mother. This is the same movie as File S1 but with only the DNA channel shown in black and white for easier viewing of the B chromosomes. (.avi, 1 MB)
  • File S3: Script modeling the B chromosome sibling cross experiment. (.doc, 36 KB)
  • File S4: Script modeling the B chromosome outcross experiment. (.doc, 32 KB)
  • Table S1: X and 4th chromosome nondisjunction in females with achiasmate Xs. (PDF, 144 KB)