SRC Homology 2 Domain-containing Leukocyte Phosphoprotein of 76 kDa (SLP-76) N-terminal Tyrosine Residues Regulate a Dynamic Signaling Equilibrium Involving Feedback of Proximal T-cell Receptor (TCR) Signaling

Supplemental Data

Files in this Data Supplement:

• Figure S1-3, S5-7 - Description of Supplemental Tables and Figures and Six Supplemental Figures.
• Figure S4 - Annotated MSMS spectra for all PTM containing peptides.
• Supplemental Table 1 - Complete list of sequence and phosphorylation site assignments of all identified phosphopeptides with corresponding SIC peak areas and statistics, protein accession numbers from NCBI nr, uniprot and HPRD, and gene ontology and KEGG functional annotation.
• Supplemental Table 2 - Complete list of phosphopeptides detected from every replicate and timepoint of TCR stimulation.
• Supplemental Table 3 - Summary of the number of phosphopeptides and phosphotyrosine sites identified from each LCMS run and the percentage of peptide SIC peak areas calculated from MSMS retention times.
• Supplemental Table 4 - Pairwise replicate correlation for evaluation of reproducibility of SIC peak areas generated from MSMS retention times or retention time alignment.