Metabolomics reveal 1-palmitoyl lysophosphatidylcholine production by peroxisome proliferator-activated receptor α

Supplemental Data

Files in this Data Supplement:

  • Supplemental Table 1 - Characterization of plasma metabolites that were influenced by PPARα activation in mice treated with bezafibrate.
  • Supplemental Table 2 - Characterization of liver metabolites that were influenced by PPARα activation in mice treated with bezafibrate.
  • Supplemental Figure 1 - Identification of LPC(16:0) in mouse plasma by LC-MS. (A) Rt data, (B) full mass data, and (C) MS/MS data of LPC(16:0). (a) authentic sample, (b) control plasma sample, (c) treatment bezafibrate plasma sample. The first elution peak (Rt = 33 min) is 2-palmitoyl lysophosphatidylcholine, the second elution peak (Rt = 34 min) is 1-palmitoyl lysophosphatidylcholine. (D) the structure of LPC(16:0) and the cleavage pattern of its fragmentation. [M]+; H+ adduct ion. MW; molecular weight.
  • Supplemental Figure 2 - The effect of siRNA transfection on Pla2g7 expression level. Data are mean ? SEM (n = 5-7). Cont; control (non-transfection cells), Ctrl siRNA; control siRNA, Pla2 siRNA; Pla2g7 siRNA