Diversity of Methane-Cycling Archaea in Hydrothermal Sediment Investigated by General and Group-Specific PCR Primers

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 -

  Supplemental methods and results, modeled temperatures and measured sulfate concentrations (Table S1), overview of cultured methanogen species (Table S2), sequence similarities (Table S3), habitat types of closest BLAST hit sequences from outside the Guaymas Basin (Table S5), gene alignments and gene sections used for distance matrix calculations (Fig. S1), scatter plot (Fig. S2), sequence similarity across taxonomic divisions (Fig. S3), and archaeal 16S rRNA gene phylogeny (Fig. S4).

  PDF, 5.3M

• Supplemental file 2 -

  Final distance matrix applied to representatives of all cultured genera of methane-cycling archaea, in addition to all noncultured environmental groups, clusters, and subclusters detected in the study (Table S4).

  XLSX, 58K