Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie01 - Movie S1. Assembly of branched actin filament networks visualized by TIRF microscopy (corresponds to Figure 1A). Reactions contained 1 μM G-actin (10% Oregon green-labeled), 10 nM Arp2/3 complex, and 100 nM GST-VCA from WAVE1, WAVE2, or N-WASP. Scale bar, 10 μm.
- Movie03 - Movie S3. Assembly of branched actin filament networks visualized by TIRF microscopy (corresponds to Figure S3B and C). Reactions contained 1 μM G-actin (10% Oregon greenlabeled), 10 nM Arp2/3 complex, and 100 nM GST-VCA chimeras between different portions of WAVE1 and N-WASP.
- Movie04 - Movie S4. TIRF microscopy showing that WAVE1 inhibits filament elongation even in the presence of excess WAVE2 (corresponds to Figure S5). Reactions contain 1 μM G-actin (10% Oregon green-labeled), 5 nM Arp2/3 complex with 100 nM WAVE1 GST-VCA and/or 500 nM WAVE2 GST-VCA.
- Movie02 - Movie S2. Elongation of unbranched actin filaments visualized by TIRF microscopy (corresponds to Figure 2A). Reactions contained 1 μM G-actin (10% Oregon green-labeled) +/- 100 nM GST-VCA from WAVE1, WAVE2, or N-WASP.