Transcriptional Analysis of Deinococcus radiodurans Reveals Novel Small RNAs That Are Differentially Expressed under Ionizing Radiation

Supplemental material

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  Deep sequencing read distribution (Fig. S1), expression of the Y RNA confirmed with the deep-seq data (Fig. S2), RT-PCR for sRNAs identified in D. radiodurans (Fig. S3), RT-PCR for sRNA cotranscription verification (Fig. S4), selected examples of transcription starting sites identified by 5’ Deep-RACE (Fig. S5), Venn diagram of computational predicted sRNA candidates and deep-sequencing identified sRNA candidates (Fig. S6), cell survival rate after irradiation (Fig. S7), Northern blots (Fig. S8), expression of DR_A0234 confirmed with the deep sequencing analysis (Fig. S9), predicted sRNAs (Table S1 and S6), probes for Northern blotting analysis (Table S2), RT-PCR primers (Table S3), probes for 5' RACE (Table S4), potential sRNAs identified with deep sequencing and computational prediction (Table S5), and target RNA prediction (Table S7).

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