Genetic Engineering Activates Biosynthesis of Aromatic Fumaric Acid Amides in the Human Pathogen Aspergillus fumigatus

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  Genetic manipulation of A. fumigatus (Fig. S1), reconstitution of the full-length ftpA reading frame to create the pET28a-based gene expression construct pDK15 (Fig. S2), SDS-polyacrylamide gel of Aspergillus fumigatus full-length FtpA after purification on Ni²⁺-NTA (Fig. S3), ¹H NMR spectra of synthetic fumaryl-L-phenylalanine and fumaryl-L-tyrosine standards (Fig. S4), oligonucleotide primers (Table S1), compounds used in substrate pools and/or individually (Table S2), and adenylation activities (Table S3).

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