Use of mCherry Red Fluorescent Protein for Studies of Protein Localization and Gene Expression in Clostridium difficile

Supplemental material

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• Supplemental file 1 -

  Codon usage in mCherryOpt (Table S1); DNA sequence of mCherryOpt (Fig. S1); Clustal Omega alignment of ZapA orthologs from different bacteria (Fig. S2); mCherryOpt-ZapA_Cd localizes to division sites (Fig. S3); induction of P_pdaV::mCherryOpt as measured by fluorescence and qRT-PCR (Fig. S4).

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