Divergent Targets of Aspergillus fumigatus AcuK and AcuM Transcription Factors during Growth In Vitro versus Invasive Disease

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 -

  Fig. S1. Relative acuM and acuK mRNA levels in mutants lacking acuK and/or acuM. Fig. S2. Deletion of acuK and acuM has no effect on the surface expression of galactosaminogalactan (GAG). Fig. S3. Deletion of acuK and acuM has no effect on cell wall β-glucan. Fig. S4. Effects of overexpression of acuM on the growth of the ΔacuK mutant on gluconeogenic carbon sources and damage to a pulmonary epithelial cell line.

  PDF, 271K

• Supplemental file 2 -

  Table S1. PCR primers used for real-time PCR and strain construction and verification.

  XLS, 46K

• Supplemental file 3 -

  Table S2. In vitro and in vivo gene expression ratios for the ΔacuK and ΔacuM mutants as compared to the wild-type strain.

  XLSX, 22K

• Supplemental file 4 -

  Table S3. Gene expression ratio for the ΔacuK gpdA-acuM overexpression mutants as compared to the ΔacuK mutant.

  XLSX, 106K