Biosynthesis of Cell Envelope-Associated Phenolic Glycolipids in Mycobacterium marinum

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 -

  Table S1, strains

  Table S2, plasmids

  Table S3, primer and amplicon information for mutant screening/verification

  Table S4, primer and amplicon information for mutagenesis and expression constructs

  Fig. S1, mutagenesis cassette delivery suicide vectors for construction of M. marinum mutants

  Fig. S2, mutant verification analysis

  Fig. S3, SDS-PAGE analyses of purified PpsA and FadD29 proteins

  Fig. S4, s-PHPA synthesis

  Supplemental text describing large-scale synthesis of s-PHPA and supporting NMR spectra

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