Metabolic Engineering of Corynebacterium glutamicum for Methanol Metabolism

Supplemental material

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• Supplemental file 1 -

  Supplemental materials and methods, oligonucleotides used in the study (Table S1), growth of recombinant C. glutamicum strains (Fig. S1), labeling fractions of the m+1 mass isotopomers of selected intracellular metabolites and amino acids for the strains C. glutamicum Ptuf-Bm(mdh-act) Ptuf-Bs(hps-phi) (Fig. S2), C. glutamicum Δald ΔadhE Ptuf-Bm(mdh-act) Ptuf-Bs(hps-phi) (Fig. S4), and C. glutamicum Δald ΔadhE Ptuf-Bm(mdh3-act) Ptuf-Bs(hps-phi) (Fig. S5), and methanol consumption and ¹³CO₂ formation (Fig. S3).

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