PolyQ-dependent RNA–protein assemblies control symmetry breaking

Files in this Data Supplement:

• Supplemental Material (PDF)
• Video 3 -
  Hyphal growth pauses and restarts in whi3Δ. Phase images were taken every 10 min for 10 h using a time-lapse wide-field microscope (AxioImager-M1; Carl Zeiss). An arrowhead appears when growth pauses.
• Video 2 -
  Isotropic growth of whi3Δ at 30°C. A whi3Δ hypha (phase image by AxioImager-M1) grows out from a spore on a microscopic slide with an agarose gel pad. Frames were taken every 10 min for 24 h.
• Macros (Zipped) - All MATLAB codes and ImageJ macros are available in a single zip file.
• Video 1 -
  Concentrated Whi3 on polarization sites. Whi3-GFP (gray) was imaged in A. gossypii using a time-lapse wide-field microscope (AxioImager-M1; Carl Zeiss). Frames were taken every 3 min for 30 min. Asterisks indicate concentrated Whi3. Arrowheads indicate emergence of a new branch.