Supplemental Data
Files in this Data Supplement:
- Supplementary Figure S1, S2, S3, S4; Supplementary Note 1 - Supplementary Fig. S1. Chemical structures of detergents and additives relevant to this study. Supplementary Fig. S2. Protein purification method development: screening of detergent and filter size. Supplementary Fig. S3. Reaction condition screening for PNGase F digestion identified enzyme concentration as a key parameter to complete GABAAR deglycosylation. Supplementary Fig. S4. Representative MS/MS spectra for the identification of deamidation from in-gel trypsin-chymotrypsin digestion of PNGase F-treated GABAAR. Supplementary Note 1. Detailed methods of [3H]radioligand binding assays.
- Supplementary Table S1, S2, S3 - Supplementary Table S1. Proteins identified from deep in-gel analysis of DDM/CHS-based affinity-purified GABAAR by searching against the human proteome and CRAPome. Supplementary Table S2. Proteins identified from deep in-gel analysis of PNGase F-treated DDM/CHS-based affinity-purified GABAAR by searching against the human proteome and CRAPome. Supplementary Table S3. Proteins identified from direct pepsin digestion and analysis of DDM/CHS-based affinity-purified GABAAR by searching against the human proteome (PD 1.3, peptide FDR<1%) and CRAPome.
- Supplementary Table S4a, S4b, S4c - Supplementary Table S4. Peptide identification lists.