LOCUS pAMG275 11776 bp DNA circular 28-FEB-2014 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|690908459| COMMENT VNTDBDATE|690908459| COMMENT LSOWNER| COMMENT VNTNAME|pAMG275| COMMENT VNTAUTHORNAME|Adam Guss| COMMENT VNTAUTHORTEL|865-576-0814| COMMENT VNTAUTHOREML|gussam@ornl.gov| FEATURES Location/Qualifiers misc_recomb 8784..9834 /vntifkey="86" /label=del\adhE#1\downstream\PCR misc_feature 7753..8783 /vntifkey="21" /label=del\adhE#1\upstream\PCR CDS 9561..9806 /locus_tag="Cthe_0425" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_001036856.1" /db_xref="GI:125972946" /db_xref="GeneID:4808428" /vntifkey="4" /label=Cthe0425-hfsA /note="KEGG: tma:TM1420 hypothetical protein" gene 9561..9806 /locus_tag="Cthe_0425" /db_xref="GeneID:4808428" /vntifkey="60" CDS 8799..9548 /locus_tag="Cthe_0424" /codon_start=1 /transl_table=11 /product="aminoglycoside phosphotransferase" /protein_id="YP_001036855.1" /db_xref="GI:125972945" /db_xref="InterPro:IPR000687" /db_xref="InterPro:IPR000719" /db_xref="InterPro:IPR002575" /db_xref="GeneID:4808427" /vntifkey="4" /label=Cthe0424-putative\kinase /note="PFAM: protein of unknown function RIO1; aminoglycoside phosphotransferase KEGG: bha:BH0326 hypothetical protein" gene 8799..9548 /locus_tag="Cthe_0424" /db_xref="GeneID:4808427" /vntifkey="60" CDS 8100..8783 /locus_tag="Cthe_0422" /codon_start=1 /transl_table=11 /product="redox-sensing transcriptional repressor Rex" /protein_id="YP_001036853.1" /db_xref="GI:125972943" /db_xref="InterPro:IPR003781" /db_xref="InterPro:IPR009718" /db_xref="GeneID:4808425" /vntifkey="4" /label=Cthe0422-putative\redox\transcription\factor /note="modulates transcription in response to the NADH/NAD(+) redox state, putatively binds CoA" gene 8100..8783 /locus_tag="Cthe_0422" /db_xref="GeneID:4808425" /vntifkey="60" CDS 11163..11708 /locus_tag="or0940" /product="hypoxanthine phosphoribosyltransferase" /EC_number="2.4.2.8" /codon_start=1 /transl_table=11 /colour=16 /vntifkey="4" /label=hpt\(putative) /note="TIGRFAM: hypoxanthine phosphoribosyltransferase PRIAM: Hypoxanthine phosphoribosyltransferase PFAM: phosphoribosyltransferase SPTR: Q3CID6 Hypoxanthine phosphoribosyl transferase (EC 2.4.2.8). Thermoanaerobacter pseudethanolicus ATCC 33223. KEGG: tpd:Teth39_0300 hypoxanthine phosphoribosyltransferase COGs: COG0634 Hypoxanthine-guanine phosphoribosyltransferase category=F InterPro IPR000836:IPR002375:IPR005904" gene 11163..11708 /locus_tag="or0940" /colour=16 /vntifkey="60" promoter complement(2824..3444) /vntifkey="30" /label=P-cbp /note="cbp promoter" rep_origin 3445..4979 /vntifkey="33" /label=putative\minimal\C.\thermocellum\ori /note="region of pNW33N" gene complement(3520..4524) /gene="repB" /vntifkey="60" CDS complement(3520..4524) /gene="repB" /codon_start=1 /transl_table=11 /product="RepB" /protein_id="AAO86061.1" /db_xref="GI:29650180" /vntifkey="4" /label=repB /note="replication protein; rolling circle replication initiation protein; Bacillus origin of replication from Geobacillus stearothermophilus plasmid pTHT15" CDS complement(4653..4901) /vntifkey="4" /label=putative\mob/pre\fragment /note="cds homologous to a fragment of mob/pre plasmid proteins involved in mobilization and recombination." repeat_region 4613..4978 /vntifkey="34" /label=pNW33N\Repeat\1 primer 9847..9866 /vntifkey="27" /label=ANTISENSE_PRM /note="Antisense primer used for creating P-gapD-cat" primer complement(11125..11145) /vntifkey="27" /label=SENSE_PRM /note="Sense primer used for creating P-gapD-cat" promoter 9847..10494 /vntifkey="30" /label=P-gapD /note="gapD promoter from C. thermocellum" CDS 10495..11145 /vntifkey="4" /label=cat terminator 1074..1327 /vntifkey="43" /label=CYC1\term rep_origin 1511..2184 /vntifkey="33" /label=pUC\origin CDS complement(5082..5942) /vntifkey="4" /label=bla /note="b-lactamase = ampR" CDS complement(5960..7067) /vntifkey="4" /label=URA3 /note="yeast gene URA3, which results in ura+, 5-FOA(Sensitive) when present" rep_origin 7080..7598 /vntifkey="33" /label=CEN6/ARSH4 /note="Yeast origin of replication" CDS complement(2245..2823) /locus_tag="or0326" /product="Thymidine kinase" /EC_number="2.7.1.21" /codon_start=1 /transl_table=11 /colour=16 /vntifkey="4" /label=tdk /note="CDS 326 - PRIAM: Thymidine kinase PFAM: thymidine kinase SPTR: Q3CIU6 Thymidine kinase (EC 2.7.1.21). Thermoanaerobacter pseudethanolicus ATCC 33223. KEGG: tpd:Teth39_2112 thymidine kinase COGs: COG1435 Thymidine kinase category=F InterPro IPR001267" gene complement(2245..2823) /locus_tag="or0326" /colour=16 /vntifkey="60" misc_feature 11741..11744 /vntifkey="21" /label=loop\of\t1t2 misc_recomb 1..1037 /vntifkey="86" /label=adhE\internal /note="internal fragment of adhE for deletion in Ct27405/1313" CDS 11163..11708 /locus_tag="or0940" /product="hypoxanthine phosphoribosyltransferase" /EC_number="2.4.2.8" /codon_start=1 /transl_table=11 /colour=16 /vntifkey="4" /label=hpt /note="From Thermoanaerobacterium saccharolyticum - TIGRFAM: hypoxanthine phosphoribosyltransferase PRIAM: Hypoxanthine phosphoribosyltransferase PFAM: phosphoribosyltransferase SPTR: Q3CID6 Hypoxanthine phosphoribosyl transferase (EC 2.4.2.8). Thermoanaerobacter pseudethanolicus ATCC 33223. KEGG: tpd:Teth39_0300 hypoxanthine phosphoribosyltransferase COGs: COG0634 Hypoxanthine-guanine phosphoribosyltransferase category=F InterPro IPR000836:IPR002375:IPR005904" BASE COUNT 3555 a 2338 c 2321 g 3562 t ORIGIN 1 tggttaatac gccgtcatcc cagggaggta tcggtgacct ttacaacttc aagcttgctc 61 cgtctctcac cctcggctgc ggttcctggg gaggaaattc agtttccgac aatgtgggag 121 tcaagcattt gttaaacatt aaaacagttg ccgagaggag agagaacatg ctctggttca 181 gaacacctga aaagatttat ataaaaagag gttgtctgcc tgttgcattg gatgagctta 241 aaaatgtaat gggtaaaaag aaagcattta ttgtaacgga taacttcctg tacaataacg 301 gctacaccaa gccgattacg gataagctgg atgaaatggg aattgtgcac aagaccttct 361 ttgatgtgtc tccagaccca tcccttgcat ctgccaaagc cggtgcggca gaaatgctgg 421 ctttccagcc tgacaccata attgcggtcg gcggcggatc tgccatggac gcggccaaaa 481 tcatgtgggt gatgtatgaa catccggaag ttgactttat ggacatggca atgagattta 541 tggatataag aaagagagtt tacaccttcc cgaagatggg acagaaggca tactttatcg 601 caattccgac ttccgcgggt acaggttcag aagtgacacc ttttgcggtt attactgatg 661 aaaaaacagg aattaaatac cctctggccg actatgaatt gttgccggac atggctattg 721 tagatgccga tatgatgatg aatgctccaa agggacttac cgcagcttcc ggtatagacg 781 cattgaccca cgctctggaa gcctatgttt caatgcttgc gaccgactat acggatagcc 841 ttgcccttcg tgcaataaag atgatatttg aatatctccc gagagcctat gaaaacggtg 901 caagtgaccc ggttgcaaga gagaaaatgg ccaatgccgc aacaatagcc ggaatggctt 961 ttgccaatgc ctttttgggt gtatgccatt caatggcgca caaactgggt gctttttatc 1021 acctgcccca cggtgttcct cagcgtttaa acccgctgat cctagagggc cgcatcatgt 1081 aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta accgaaaagg 1141 aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat 1201 taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat 1261 gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt 1321 gcaagctgcg gccctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 1381 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 1441 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 1501 aggaaagaac atgtgagcaa aaggccagca aaagcccagg aaccgtaaaa aggccgcgtt 1561 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 1621 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 1681 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 1741 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 1801 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 1861 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 1921 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 1981 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 2041 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 2101 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 2161 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 2221 gattttggtc atgaaaaaat ccgcttaagt ccgcgggact tcatggcact ttctacacct 2281 tgcttcataa gactcctttg ctccaaccat tataatggga tcgtcgtaat tggcaggctt 2341 cccatttata agcctttgcg tgcgagtagc aggattgcca cacttcatgc atatagcagt 2401 aagcttatcg acaaattcag ctatggccat caattctgga gtcggaccaa atggttcacc 2461 tctaaagtcc atgtcaagcc ctgcacatat gactcttttc ccgctatcgg caatctcttt 2521 tacgatgtcg actatttcag aatcaaaaaa ttgaacttca tctatagcaa atacatccgt 2581 atcttcttca gcataagcca atatgtcaga agcctttact atggcaatgg cgtgcatgtt 2641 gtcgccgtta tgagatacga ctttgtctat ggaataccta tcgtctatag ccggtttgaa 2701 aacctgaact ttttgcctgg caatcttagc tctctttatc cttcttataa gttcctcact 2761 ttttccactg aacatgggac cagttacaac ttctatgtac ccgtggtctt taggcccata 2821 catctttcgt cctccttaaa attttcgttt atttgttttt tatttagtgt gcctaaaagc 2881 accatcttat acattcgtac cttatatctc ttatcttgta tctcttacct catatctctt 2941 attattaact accgaagtat attttcataa cggaatctta tgacttattt ttctgactgc 3001 cgttttacca tacggttaat ttatacggaa acagctaatc taaccttgct gaaggcatgt 3061 ttaccaaaat attatatcta ctttaccata ttaattcaag tggtcatttt gatattttac 3121 gccaaaattt ttagcgttcc tttacttgat aatttatcca aaaaccttca ttcgtcaaaa 3181 caattatgat ataattaatt tatgatatga ttaattaata tttacggata actaatcatt 3241 tcaatgcaat agatcaacat gcaaatcaat atccacctta ctccgaacat tttgcaccaa 3301 cgaatgccca gcactatatc ccggcaagca atataacgtc tataattttt tatgataacg 3361 ataaaattaa attttatgaa aggggtaaaa gcatgagaaa aatagctgta ttgttaatta 3421 ctttgacgtt cttagtcacg actcgaattt aggaggctta cttgtctgct ttcttcatta 3481 gaatcaatcc ttttttaaaa gtcaatcccg tttgttgaac tactctttaa taaaataatt 3541 tttccgttcc caattccaca ttgcaataat agaaaatcca tcttcatcgg ctttttcgtc 3601 atcatctgta tgaatcaaat cgccttcttc tgtgtcatca aggtttaatt ttttatgtat 3661 ttcttttaac aaaccaccat aggagattaa ccttttacgg tgtaaacctt cctccaaatc 3721 agacaaacgt ttcaaattct tttcttcatc atcggtcata aaatccgtat cctttacagg 3781 atattttgca gtttcgtcaa ttgccgattg tatatccgat ttatatttat ttttcggtcg 3841 aatcatttga acttttacat ttggatcata gtctaatttc attgcctttt tccaaaattg 3901 aatccattgt ttttgattca cgtagttttc tgtattctta aaataagttg gttccacaca 3961 taccaataca tgcatgtgct gattataaga attatcttta ttatttattg tcacttccgt 4021 tgcacgcata aaaccaacaa gatttttatt aattttttta tattgcatca ttcggcgaaa 4081 tccttgagcc atatctgaca aactcttatt taattcttcg ccatcataaa catttttaac 4141 tgttaatgtg agaaacaacc aacgaactgt tggcttttgt ttaataactt cagcaacaac 4201 cttttgtgac tgaatgccat gtttcattgc tctcctccag ttgcacattg gacaaagcct 4261 ggatttacaa aaccacactc gatacaactt tctttcgcct gtttcacgat tttgtttata 4321 ctctaatatt tcagcacaat cttttactct ttcagccttt ttaaattcaa gaatatgcag 4381 aagttcaaag taatcaacat tagcgatttt cttttctctc catggtctca cttttccact 4441 ttttgtcttg tccactaaaa cccttgattt ttcatctgaa taaatgctac tattaggaca 4501 cataatatta aaagaaaccc ccatctattt agttatttgt ttggtcactt ataactttaa 4561 cagatggggt ttttctgtgc aaccaatttt aagggttttc aatactttaa aacacataca 4621 taccaacact tcaacgcacc tttcagcaac taaaataaaa atgacgttat ttctatatgt 4681 atcaagaata gaaagaactc gtttttcgct acgctcaaaa cgcaaaaaaa gcactcattc 4741 gagtgctttt tcttatcgct ccaaatcatg cgattttttc ctctttgctt ttctttgctc 4801 acgaagttct cgatcacgct gcaaaacatc ttgaagcgaa aaagtattct tcttttcttc 4861 cgatcgctca tgctgacgca cgaaaagccc tctaggcgca taggaacaac tcctaaatgc 4921 atgtgagggg ttttctcgtc catgtgaaca gtcgcatacg caatattttg tttcccatag 4981 cggccgcgat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 5041 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 5101 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 5161 gtgtagataa ctacgatacg ggagcgctta ccatctggcc ccagtgctgc aatgataccg 5221 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 5281 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 5341 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgg cattgctaca 5401 ggcatcgtgg tgtcactctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 5461 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 5521 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 5581 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 5641 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 5701 cgggataata gtgtatcaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 5761 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 5821 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 5881 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 5941 atactcttcc tttttcaatg ggtaataact gatataatta aattgaagct ctaatttgtg 6001 agtttagtat acatgcattt acttataata cagtttttta gttttgctgg ccgcatcttc 6061 tcaaatatgc ttcccagcct gcttttctgt aacgttcacc ctctacctta gcatcccttc 6121 cctttgcaaa tagtcctctt ccaacaataa taatgtcaga tcctgtagag accacatcat 6181 ccacggttct atactgttga cccaatgcgt ctcccttgtc atctaaaccc acaccgggtg 6241 tcataatcaa ccaatcgtaa ccttcatctc ttccacccat gtctctttga gcaataaagc 6301 cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt acccttagta tattctccag 6361 tagataggga gcccttgcat gacaattctg ctaacatcaa aaggcctcta ggttcctttg 6421 ttacttcttc tgccgcctgc ttcaaaccgc taacaatacc tgggcccacc acaccgtgtg 6481 cattcgtaat gtctgcccat tctgctattc tgtatacacc cgcagagtac tgcaatttga 6541 ctgtattacc aatgtcagca aattttctgt cttcgaagag taaaaaattg tacttggcgg 6601 ataatgcctt tagcggctta actgtgccct ccatggaaaa atcagtcaag atatccacat 6661 gtgtttttag taaacaaatt ttgggaccta atgcttcaac taactccagt aattccttgg 6721 tggtacgaac atccaatgaa gcacacaagt ttgtttgctt ttcgtgcatg atattaaata 6781 gcttggcagc aacaggacta ggatgagtag cagcacgttc cttatatgta gctttcgaca 6841 tgatttatct tcgtttcctg caggtttttg ttctgtgcag ttgggttaag aatactgggc 6901 aatttcatgt ttcttcaaca ctacatatgc gtatatatac caatctaagt ctgtgctcct 6961 tccttcgttc ttccttctgt tcggagatta ccgaatcaaa aaaatttcaa agaaaccgaa 7021 atcaaaaaaa agaataaaaa aaaaatgatg aattgaattg aaaagctagc ttatcgatgg 7081 gtccttttca tcacgtgcta taaaaataat tataatttaa attttttaat ataaatatat 7141 aaattaaaaa tagaaagtaa aaaaagaaat taaagaaaaa atagtttttg ttttccgaag 7201 atgtaaaaga ctctaggggg atcgccaaca aatactacct tttatcttgc tcttcctgct 7261 ctcaggtatt aatgccgaat tgtttcatct tgtctgtgta gaagaccaca cacgaaaatc 7321 ctgtgatttt acattttact tatcgttaat cgaatgtata tctatttaat ctgcttttct 7381 tgtctaataa atatatatgt aaagtacgct ttttgttgaa attttttaaa cctttgttta 7441 tttttttttc ttcattccgt aactcttcta ccttctttat ttactttcta aaatccaaat 7501 acaaaacata aaaataaata aacacagagt aaattcccaa attattccat cattaaaaga 7561 tacgaggcgc gtgtaagtta caggcaagcg atccgtccgc cggcgaacgt ggcgagaaag 7621 gaagggaaga aagcgaaagg agcgggggct agggcggtgg gaagtgtagg ggtcacgctg 7681 ggcgtaacca ccacacccgc cgcgcttaat ggggcgctac agggcgcgtg gggatgatcc 7741 actagtaagc ttgcagattc agccgatact tattaaatat tgattagtct gagacttaaa 7801 ccggttttaa taagaagcag gagctaaaat tttagcttcc gcttttttta ttttctcaga 7861 aaatttattt ttgtgaaaac tatataaaac tttcattcaa attgatatga aaatgtataa 7921 aaagtgtatt gactttgatt gtattaaacg gtaaaatgat atcgataaca aaatatcgat 7981 aaaaaaatac cgataaataa atatcgatat caataatctt atagtacttg agttaaaagg 8041 ggtggattgg ctaaaatagt ttaataagta attattaaaa aagaaaggaa ccaatatata 8101 tgaataatcc gaaagctatc tcaaaacaga ctcttttaag attaccttca tatttaagtt 8161 acttaaaatc gctgccaaag caggacggtg aatatgtatc ggctacaatg attgcttcgg 8221 cgctgggact gaatgatgtg caagttcgaa aggatctggc ctgtgtaagc aaaaaaggcc 8281 gtccaaagct tggatatgtt gctgaagagt tgataaggga tatagagagt tttcttgggt 8341 atgactcttt gaacgatgcg ataattgtgg gcgcgggaag gcttggagga gctcttttat 8401 cctatgaggg ctttaaagaa tatggattaa atattgttgc agcgtttgat attgatgaaa 8461 gtaaaatcgg cactgaaatt tgcgggagaa aaattttccc tttggacaaa atgaaggaac 8521 tgtgccggcg gatgaaaatt cgtatcggta taataaccgt ccctgcagat tcagcccaaa 8581 aagtatgcga catgcttgta gacagcggaa tatatgcaat atggaatttt gcacctgttc 8641 accttaaagt accggataac attttagtcc agcaagaaaa catggcggca tcactggctg 8701 tactttccgc acacttggca gaagcaataa agaataatgg aatcggaaaa ggagatgagg 8761 aaaatgacga aaatagcgaa taaaggaggt gcgaagaaat gaagcttgaa aaggttatag 8821 cggtaagaac ggacaaaacc gtttacagag acggagacaa ggcaataaag gtcttttcca 8881 gcaactattc aaaggccaac gtattgaatg aagctttaaa ccaggccagg gtggaagaaa 8941 ccgggcttaa cattccgaag cttcttgaag ttacaaaaat tgatggcaaa tgggcaattg 9001 tgacggagtt tattgaagga aagaccttag agcagcttat gaaagaaaat cccttaaaat 9061 atgatgaata tatgaacctc tttgttgata ttcagctcga agtgttaagc aaaagggctc 9121 ctttattaaa caagctgaaa gacaaaatgc acagaaaaat ctcagagagc ggtcttgatg 9181 ccacgacgcg ctatgagctt catacaagac ttcaaagtat gcccgaccat aacaaggtgt 9241 gtcatggtga tttcaatccc agcaatatta ttataaccaa agacggcact ccttatatac 9301 ttgactggtc gcacgtcacc cagggaaacg ccagcgccga tgccgcgaga acatacctgt 9361 tgttctggct ttccggcgat attgacaagg cagccaagta tttgaacctt ttctgtaaaa 9421 agagcgacac tgcaaagcaa tatgttcaga agtggatttc aattgtagcg gcatcccagc 9481 ttgtaaaagg aagagaagct gaaagggagt tattaatgcg ctggattgat gttgtagatt 9541 acgaatagga ggtatttggg atgaaagtgt ctatttgcat agggagctct tgccacttaa 9601 aaggagcaaa gcagatagtg gaacagctgc aaagcctggt tgccgactat aacctgaaag 9661 agaaagttga acttggcggg gcattttgta tgaaaaactg tgttaacggt gtcagcgtga 9721 cagttgatga taaactgttt tctgtcactc cggaaaacgt gaaaagcttt tttgaaacag 9781 agattcttaa aaaacttgag gactgaaaag taaaaaacaa ataagcggtg ctgctcgcga 9841 ggccggccag tattctgaca tgggtgtatc aataacccat gcgtttccgt attgtatcgg 9901 aatggtttcg gacagggcgg tgggaataga catggaaaag atttttttgc ccgaggatgc 9961 attgataaag tatttctttt ccgaaagaga ggaaaagatt ctaaagagtt ttggaaatac 10021 tgatgaatat tgtgtgcaga gtacaattct atggacaaga aaagaggctt tgtcaaaact 10081 ttttcgtctg ggaatgagga tggattttaa aaagctggat actttggagg acgaggtggt 10141 ttttcaggaa acaaacaggg cgcgtctgtt ttcttttata tgcaataatt actgtatctc 10201 tctggcattg ccaggtttta ataaagatta aaattattga ctagaaataa aaaaattgtc 10261 cataatatta atggacaaaa aaacaaagaa ttacatcaaa ggaagataaa aatactttgt 10321 taaaaaatta attatttttt atctaaacta ttgaaaatga aaataaaata atataaaatg 10381 aatcatagtg caagagatac ttgccagagg atgaatattt tactgcattc atgctttatg 10441 gcagctaata gaggcattaa attaaatttt aatttacaat aggaggcgat attaatgaac 10501 tttaataaaa ttgatttaga caattggaag agaaaagaga tatttaatca ttatttgaac 10561 caacaaacga cttttagtat aaccacagaa attgatatta gtgttttata ccgaaacata 10621 aaacaagaag gatataaatt ttaccctgca tttattttct tagtgacaag ggtgataaac 10681 tcaaatacag cttttagaac tggttacaat agcgacggag agttaggtta ttgggataag 10741 ttagagccac tttatacaat ttttgatggt gtatctaaaa cattctctgg tatttggact 10801 cctgtaaaga atgacttcaa agagttttat gatttatacc tttctgatgt agagaaatat 10861 aatggttcgg ggaaattgtt tcccaaaaca cctatacctg aaaatgcttt ttctctttct 10921 attattccat ggacttcatt tactgggttt aacttaaata tcaataataa tagtaattac 10981 cttctaccca ttattacagc aggaaaattc attaataaag gtaattcaat atatttaccg 11041 ctatctttac aggtacatca ttctgtttgt gatggttatc atgcaggatt gtttatgaac 11101 tctattcagg aattgtcaga taggcctaat gactggcttt tataataaag gaggtcgacg 11161 tcatggaaaa tttatcaaaa gacatcgatg aaattttgat cacagaagaa gaacttaagg 11221 aaaagataaa agagcttggg aggcaaatca caaaagacta caaagggaaa aatttgatgt 11281 tggtaggagt tttaaaaggt gctttaatgt ttatggctga tttgtcaaga cacatagatt 11341 tgcctttatc acttgatttt atggctgttt ccagctatgg aagctcaact cattcatcag 11401 gaatagtaaa gataatcaaa gatcttgata taagcataga aggcaaagat gttctgattg 11461 tggaagacat aattgacagc ggtttgactt tgtcttactt aagggaaact ttacttggaa 11521 ggaagccaaa aagcctgaaa atatgcacaa tattagacaa accggagaga agagaagcat 11581 ctgtaaaagt cgattatgta ggatttaaga tacctgataa gtttgtcgtg ggttatggat 11641 tggactttga tgaaaagtac aggaaccttc cttttatagg cgttttgaaa cctgaaatgt 11701 acagctaagc ccgggcctcg agaaaacaaa aggctcagtc ggaagactgg gccttttgtt 11761 ttggtaccga attcgg // LOCUS pAMG278 11694 bp DNA circular 28-FEB-2014 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|690908667| COMMENT VNTDBDATE|690908667| COMMENT LSOWNER| COMMENT VNTNAME|pAMG278| COMMENT VNTAUTHORNAME|Adam Guss| COMMENT VNTAUTHORTEL|865-576-0814| COMMENT VNTAUTHOREML|gussam@ornl.gov| FEATURES Location/Qualifiers gene complement(1214..1792) /locus_tag="or0326" /colour=16 /vntifkey="60" CDS complement(1214..1792) /locus_tag="or0326" /product="Thymidine kinase" /EC_number="2.7.1.21" /codon_start=1 /transl_table=11 /colour=16 /vntifkey="4" /label=tdk /note="CDS 326 - PRIAM: Thymidine kinase PFAM: thymidine kinase SPTR: Q3CIU6 Thymidine kinase (EC 2.7.1.21). Thermoanaerobacter pseudethanolicus ATCC 33223. KEGG: tpd:Teth39_2112 thymidine kinase COGs: COG1435 Thymidine kinase category=F InterPro IPR001267" rep_origin 6049..6567 /vntifkey="33" /label=CEN6/ARSH4 /note="Yeast origin of replication" CDS complement(4929..6036) /vntifkey="4" /label=URA3 /note="yeast gene URA3, which results in ura+, 5-FOA(Sensitive) when present" CDS complement(4051..4911) /vntifkey="4" /label=bla /note="b-lactamase = ampR" rep_origin 480..1153 /vntifkey="33" /label=pUC\origin terminator 43..296 /vntifkey="43" /label=CYC1\term CDS complement(3622..3870) /vntifkey="4" /label=putative\mob/pre\fragment /note="cds homologous to a fragment of mob/pre plasmid proteins involved in mobilization and recombination." CDS complement(2489..3493) /gene="repB" /codon_start=1 /transl_table=11 /product="RepB" /protein_id="AAO86061.1" /db_xref="GI:29650180" /vntifkey="4" /label=repB /note="replication protein; rolling circle replication initiation protein; Bacillus origin of replication from Geobacillus stearothermophilus plasmid pTHT15" gene complement(2489..3493) /gene="repB" /vntifkey="60" rep_origin 2414..3948 /vntifkey="33" /label=putative\minimal\C.\thermocellum\ori /note="region of pNW33N" promoter complement(1793..2413) /vntifkey="30" /label=P-cbp /note="cbp promoter" gene 6807..7043 /db_xref="GeneID:4808182" /vntifkey="60" CDS 6807..7043 /codon_start=1 /transl_table=11 /product="4-oxalocrotonate tautomerase" /protein_id="YP_001037080.1" /db_xref="GI:125973170" /db_xref="InterPro:IPR004370" /db_xref="GeneID:4808182" /locus_tag=CDS_626 /vntifkey="4" /label=Cthe_0652 /note="PFAM: 4-oxalocrotonate tautomerase KEGG: aba:Acid345_2511 4-oxalocrotonate tautomerase" gene 7171..7434 /db_xref="GeneID:4808183" /vntifkey="60" CDS 7171..7434 /codon_start=1 /transl_table=11 /product="CopG family transcriptional regulator" /protein_id="YP_001037081.1" /db_xref="GI:125973171" /db_xref="GeneID:4808183" /locus_tag=CDS_627 /vntifkey="4" /label=Cthe_0653 /note="KEGG: mta:Moth_1736 hypothetical protein" misc_feature 10646..10649 /vntifkey="21" /label=loop\of\t1t2 CDS 9400..10050 /vntifkey="4" /label=cat promoter 8752..9399 /vntifkey="30" /label=P-gapD /note="gapD promoter from C. thermocellum" primer complement(10030..10050) /vntifkey="27" /label=SENSE_PRM /note="Sense primer used for creating P-gapD-cat" primer 8752..8771 /vntifkey="27" /label=ANTISENSE_PRM /note="Antisense primer used for creating P-gapD-cat" gene 10068..10613 /locus_tag="or0940" /colour=16 /vntifkey="60" CDS 10068..10613 /locus_tag="or0940" /product="hypoxanthine phosphoribosyltransferase" /EC_number="2.4.2.8" /codon_start=1 /transl_table=11 /colour=16 /vntifkey="4" /label=hpt\(putative) /note="TIGRFAM: hypoxanthine phosphoribosyltransferase PRIAM: Hypoxanthine phosphoribosyltransferase PFAM: phosphoribosyltransferase SPTR: Q3CID6 Hypoxanthine phosphoribosyl transferase (EC 2.4.2.8). Thermoanaerobacter pseudethanolicus ATCC 33223. KEGG: tpd:Teth39_0300 hypoxanthine phosphoribosyltransferase COGs: COG0634 Hypoxanthine-guanine phosphoribosyltransferase category=F InterPro IPR000836:IPR002375:IPR005904" misc_recomb 6722..7732 /vntifkey="86" /label=hydG\up /note="upstream region of hydG (Cthe0654) for deletion" misc_recomb 7733..8739 /vntifkey="86" /label=hydG\down /note="region downstream of hydG (Cthe0654) for deletion" misc_recomb 10674..11694 /vntifkey="86" /label=hydG\internal\fragment /note="internal fragment of hydG (Cthe0654) for gene deletion" CDS 10068..10613 /locus_tag="or0940" /product="hypoxanthine phosphoribosyltransferase" /EC_number="2.4.2.8" /codon_start=1 /transl_table=11 /colour=16 /vntifkey="4" /label=hpt /note="From Thermoanaerobacterium saccharolyticum - TIGRFAM: hypoxanthine phosphoribosyltransferase PRIAM: Hypoxanthine phosphoribosyltransferase PFAM: phosphoribosyltransferase SPTR: Q3CID6 Hypoxanthine phosphoribosyl transferase (EC 2.4.2.8). Thermoanaerobacter pseudethanolicus ATCC 33223. KEGG: tpd:Teth39_0300 hypoxanthine phosphoribosyltransferase COGs: COG0634 Hypoxanthine-guanine phosphoribosyltransferase category=F InterPro IPR000836:IPR002375:IPR005904" BASE COUNT 3606 a 2235 c 2294 g 3559 t ORIGIN 1 ggcgcgcctc agcgtttaaa cccgctgatc ctagagggcc gcatcatgta attagttatg 61 tcacgcttac attcacgccc tccccccaca tccgctctaa ccgaaaagga aggagttaga 121 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta 181 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata 241 ctgaaaacct tgcttgagaa ggttttggga cgctcgaagg ctttaatttg caagctgcgg 301 ccctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 361 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 421 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 481 tgtgagcaaa aggccagcaa aagcccagga accgtaaaaa ggccgcgttg ctggcgtttt 541 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 601 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 661 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 721 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 781 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 841 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 901 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 961 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 1021 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 1081 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 1141 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 1201 tgaaaaaatc cgcttaagtc cgcgggactt catggcactt tctacacctt gcttcataag 1261 actcctttgc tccaaccatt ataatgggat cgtcgtaatt ggcaggcttc ccatttataa 1321 gcctttgcgt gcgagtagca ggattgccac acttcatgca tatagcagta agcttatcga 1381 caaattcagc tatggccatc aattctggag tcggaccaaa tggttcacct ctaaagtcca 1441 tgtcaagccc tgcacatatg actcttttcc cgctatcggc aatctctttt acgatgtcga 1501 ctatttcaga atcaaaaaat tgaacttcat ctatagcaaa tacatccgta tcttcttcag 1561 cataagccaa tatgtcagaa gcctttacta tggcaatggc gtgcatgttg tcgccgttat 1621 gagatacgac tttgtctatg gaatacctat cgtctatagc cggtttgaaa acctgaactt 1681 tttgcctggc aatcttagct ctctttatcc ttcttataag ttcctcactt tttccactga 1741 acatgggacc agttacaact tctatgtacc cgtggtcttt aggcccatac atctttcgtc 1801 ctccttaaaa ttttcgttta tttgtttttt atttagtgtg cctaaaagca ccatcttata 1861 cattcgtacc ttatatctct tatcttgtat ctcttacctc atatctctta ttattaacta 1921 ccgaagtata ttttcataac ggaatcttat gacttatttt tctgactgcc gttttaccat 1981 acggttaatt tatacggaaa cagctaatct aaccttgctg aaggcatgtt taccaaaata 2041 ttatatctac tttaccatat taattcaagt ggtcattttg atattttacg ccaaaatttt 2101 tagcgttcct ttacttgata atttatccaa aaaccttcat tcgtcaaaac aattatgata 2161 taattaattt atgatatgat taattaatat ttacggataa ctaatcattt caatgcaata 2221 gatcaacatg caaatcaata tccaccttac tccgaacatt ttgcaccaac gaatgcccag 2281 cactatatcc cggcaagcaa tataacgtct ataatttttt atgataacga taaaattaaa 2341 ttttatgaaa ggggtaaaag catgagaaaa atagctgtat tgttaattac tttgacgttc 2401 ttagtcacga ctcgaattta ggaggcttac ttgtctgctt tcttcattag aatcaatcct 2461 tttttaaaag tcaatcccgt ttgttgaact actctttaat aaaataattt ttccgttccc 2521 aattccacat tgcaataata gaaaatccat cttcatcggc tttttcgtca tcatctgtat 2581 gaatcaaatc gccttcttct gtgtcatcaa ggtttaattt tttatgtatt tcttttaaca 2641 aaccaccata ggagattaac cttttacggt gtaaaccttc ctccaaatca gacaaacgtt 2701 tcaaattctt ttcttcatca tcggtcataa aatccgtatc ctttacagga tattttgcag 2761 tttcgtcaat tgccgattgt atatccgatt tatatttatt tttcggtcga atcatttgaa 2821 cttttacatt tggatcatag tctaatttca ttgccttttt ccaaaattga atccattgtt 2881 tttgattcac gtagttttct gtattcttaa aataagttgg ttccacacat accaatacat 2941 gcatgtgctg attataagaa ttatctttat tatttattgt cacttccgtt gcacgcataa 3001 aaccaacaag atttttatta atttttttat attgcatcat tcggcgaaat ccttgagcca 3061 tatctgacaa actcttattt aattcttcgc catcataaac atttttaact gttaatgtga 3121 gaaacaacca acgaactgtt ggcttttgtt taataacttc agcaacaacc ttttgtgact 3181 gaatgccatg tttcattgct ctcctccagt tgcacattgg acaaagcctg gatttacaaa 3241 accacactcg atacaacttt ctttcgcctg tttcacgatt ttgtttatac tctaatattt 3301 cagcacaatc ttttactctt tcagcctttt taaattcaag aatatgcaga agttcaaagt 3361 aatcaacatt agcgattttc ttttctctcc atggtctcac ttttccactt tttgtcttgt 3421 ccactaaaac ccttgatttt tcatctgaat aaatgctact attaggacac ataatattaa 3481 aagaaacccc catctattta gttatttgtt tggtcactta taactttaac agatggggtt 3541 tttctgtgca accaatttta agggttttca atactttaaa acacatacat accaacactt 3601 caacgcacct ttcagcaact aaaataaaaa tgacgttatt tctatatgta tcaagaatag 3661 aaagaactcg tttttcgcta cgctcaaaac gcaaaaaaag cactcattcg agtgcttttt 3721 cttatcgctc caaatcatgc gattttttcc tctttgcttt tctttgctca cgaagttctc 3781 gatcacgctg caaaacatct tgaagcgaaa aagtattctt cttttcttcc gatcgctcat 3841 gctgacgcac gaaaagccct ctaggcgcat aggaacaact cctaaatgca tgtgaggggt 3901 tttctcgtcc atgtgaacag tcgcatacgc aatattttgt ttcccatagc ggccgcgatt 3961 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 4021 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 4081 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 4141 tacgatacgg gagcgcttac catctggccc cagtgctgca atgataccgc gagacccacg 4201 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 4261 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 4321 aagtagttcg ccagttaata gtttgcgcaa cgttgttggc attgctacag gcatcgtggt 4381 gtcactctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 4441 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 4501 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 4561 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 4621 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatag 4681 tgtatcacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 4741 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 4801 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 4861 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 4921 ttttcaatgg gtaataactg atataattaa attgaagctc taatttgtga gtttagtata 4981 catgcattta cttataatac agttttttag ttttgctggc cgcatcttct caaatatgct 5041 tcccagcctg cttttctgta acgttcaccc tctaccttag catcccttcc ctttgcaaat 5101 agtcctcttc caacaataat aatgtcagat cctgtagaga ccacatcatc cacggttcta 5161 tactgttgac ccaatgcgtc tcccttgtca tctaaaccca caccgggtgt cataatcaac 5221 caatcgtaac cttcatctct tccacccatg tctctttgag caataaagcc gataacaaaa 5281 tctttgtcgc tcttcgcaat gtcaacagta cccttagtat attctccagt agatagggag 5341 cccttgcatg acaattctgc taacatcaaa aggcctctag gttcctttgt tacttcttct 5401 gccgcctgct tcaaaccgct aacaatacct gggcccacca caccgtgtgc attcgtaatg 5461 tctgcccatt ctgctattct gtatacaccc gcagagtact gcaatttgac tgtattacca 5521 atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt acttggcgga taatgccttt 5581 agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga tatccacatg tgtttttagt 5641 aaacaaattt tgggacctaa tgcttcaact aactccagta attccttggt ggtacgaaca 5701 tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga tattaaatag cttggcagca 5761 acaggactag gatgagtagc agcacgttcc ttatatgtag ctttcgacat gatttatctt 5821 cgtttcctgc aggtttttgt tctgtgcagt tgggttaaga atactgggca atttcatgtt 5881 tcttcaacac tacatatgcg tatatatacc aatctaagtc tgtgctcctt ccttcgttct 5941 tccttctgtt cggagattac cgaatcaaaa aaatttcaaa gaaaccgaaa tcaaaaaaaa 6001 gaataaaaaa aaaatgatga attgaattga aaagctagct tatcgatggg tccttttcat 6061 cacgtgctat aaaaataatt ataatttaaa ttttttaata taaatatata aattaaaaat 6121 agaaagtaaa aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga tgtaaaagac 6181 tctaggggga tcgccaacaa atactacctt ttatcttgct cttcctgctc tcaggtatta 6241 atgccgaatt gtttcatctt gtctgtgtag aagaccacac acgaaaatcc tgtgatttta 6301 cattttactt atcgttaatc gaatgtatat ctatttaatc tgcttttctt gtctaataaa 6361 tatatatgta aagtacgctt tttgttgaaa ttttttaaac ctttgtttat ttttttttct 6421 tcattccgta actcttctac cttctttatt tactttctaa aatccaaata caaaacataa 6481 aaataaataa acacagagta aattcccaaa ttattccatc attaaaagat acgaggcgcg 6541 tgtaagttac aggcaagcga tccgtccgcc ggcgaacgtg gcgagaaagg aagggaagaa 6601 agcgaaagga gcgggggcta gggcggtggg aagtgtaggg gtcacgctgg gcgtaaccac 6661 cacacccgcc gcgcttaatg gggcgctaca gggcgcgtgg ggatgatcca ctagtaagct 6721 ttaataagga gttctatcca gcttagcggt tttaaaaagc ttttttctga tggtataaat 6781 aactaattgt cagggaggaa tacgaaatgc cgcatgttat cgttaaatta tggcccggaa 6841 ggacggaaga acaaaaacag gagcttgcaa acagaatcgc aaaggatgtc atagaaacat 6901 taaatgctcc tcaaaccagc gtttccgtaa gcattcagga agttccaaga gaaaaatggg 6961 ttgatgaagt gtataatcct gatataatgg acaaagaaga aacactttat attaagcctg 7021 gatacaaacc ggaaaatctt taaagggagt ttgtaaggga agctttgaat ataaaaagcc 7081 taaaataata tgaatttctg tttacatttc atatagattt ggtatataat taatatagtt 7141 aattttttaa cgaaggcaga agaattagaa atggagaaag aaaataggaa ggaaaaccgc 7201 atagccgtta taggaattgt aattcacaac cgtgaggaaa gttcaaggaa agtaaacgag 7261 atattgagtg attacggcaa tataattgtc ggaagaatgg gtgtgccgta caaggaaaaa 7321 ggcatttctg taatagcaat aattgttgac ggcacaactg atgaaatagg ggctcttaca 7381 ggcaagcttg gcaacataaa cggagtgaag gcaaaagttg ctttaacaaa ttaaagccct 7441 taaattaaac ttgaattaaa atgaaaattt gatattggtt tctaagatgc aggaattgtt 7501 aaaattctga catcgaggaa cgtatttatg gttttacctg acgtgacgga ttgccattta 7561 tggtaactac gggaatctga gcgaaggggg aatagatatg gtagttttgt ctataaactc 7621 ttcctgactt atgtaggtca cgaagagttt ttgtttttct gattccatat agtggaactg 7681 gcaaaaggta aatttataca atatgtgccg atacaaggtt tgtaaattga aagctgaaaa 7741 atttaagtga tattagaagc aataaactga atatatatgt atatgaaaaa agatttagaa 7801 acataaatac aatttgtttt ccggaatttg ttttcgatac ccggacattt tattacagaa 7861 tatagaacag ttggcaaact ccttggatat ttataaaaat tgtaaggatg gaataaaatg 7921 atttatcttg acaatgcggc aacttcatat ccgaagcctg acagagttta tgatgaaatg 7981 cttttatgta tgaaggaata ctgtgcaaat cccggaaggt cagggcatga actggcaata 8041 aaaacgggaa gggcggtgta tgaaacccgg gaaattgtgt caagattttt caatatcgaa 8101 aatcctatga gggtcgtatt taccaaaaat gcaacagaag ctttgaacct tgccattaac 8161 ggagtattga aagaagggga acatgtaatt actacaagca tggagcacaa ctctgtttta 8221 agacctttaa aaacgctgga gagaaacaat attatagagc ttaccatagt atggggaaat 8281 tatttcggtg aaattgatgt ggcggatata gaaagaagta taaaaaagaa cacaaaaatg 8341 attatatgtt ccctttcctc caacgttaat ggtataataa tgcccgtaaa agaaataggg 8401 aaaataacca gggaaagggg aattttattt cttgttgacg catcccaagg ggcaggttct 8461 ataaagctgg atgtccagga aataaatgca gatttgtttg ccgttccggg ccataaggat 8521 ttacttggtc cccagggagt aggtgcactt tatgtgaacg aaaatgtgga aataacgccg 8581 ataatgcagg ggggtacagg cagtcgttct gaaagttttt atcaaccgga aatattcccc 8641 gatatgctgg aaagcggaac cttgaatgct ccaggcatag tcggtttggg atttgggatt 8701 aaatttatag aaagtttcgg agttgacaat atcagaattt cgcgaggccg gccagtattc 8761 tgacatgggt gtatcaataa cccatgcgtt tccgtattgt atcggaatgg tttcggacag 8821 ggcggtggga atagacatgg aaaagatttt tttgcccgag gatgcattga taaagtattt 8881 cttttccgaa agagaggaaa agattctaaa gagttttgga aatactgatg aatattgtgt 8941 gcagagtaca attctatgga caagaaaaga ggctttgtca aaactttttc gtctgggaat 9001 gaggatggat tttaaaaagc tggatacttt ggaggacgag gtggtttttc aggaaacaaa 9061 cagggcgcgt ctgttttctt ttatatgcaa taattactgt atctctctgg cattgccagg 9121 ttttaataaa gattaaaatt attgactaga aataaaaaaa ttgtccataa tattaatgga 9181 caaaaaaaca aagaattaca tcaaaggaag ataaaaatac tttgttaaaa aattaattat 9241 tttttatcta aactattgaa aatgaaaata aaataatata aaatgaatca tagtgcaaga 9301 gatacttgcc agaggatgaa tattttactg cattcatgct ttatggcagc taatagaggc 9361 attaaattaa attttaattt acaataggag gcgatattaa tgaactttaa taaaattgat 9421 ttagacaatt ggaagagaaa agagatattt aatcattatt tgaaccaaca aacgactttt 9481 agtataacca cagaaattga tattagtgtt ttataccgaa acataaaaca agaaggatat 9541 aaattttacc ctgcatttat tttcttagtg acaagggtga taaactcaaa tacagctttt 9601 agaactggtt acaatagcga cggagagtta ggttattggg ataagttaga gccactttat 9661 acaatttttg atggtgtatc taaaacattc tctggtattt ggactcctgt aaagaatgac 9721 ttcaaagagt tttatgattt atacctttct gatgtagaga aatataatgg ttcggggaaa 9781 ttgtttccca aaacacctat acctgaaaat gctttttctc tttctattat tccatggact 9841 tcatttactg ggtttaactt aaatatcaat aataatagta attaccttct acccattatt 9901 acagcaggaa aattcattaa taaaggtaat tcaatatatt taccgctatc tttacaggta 9961 catcattctg tttgtgatgg ttatcatgca ggattgttta tgaactctat tcaggaattg 10021 tcagataggc ctaatgactg gcttttataa taaaggaggt cgacgtcatg gaaaatttat 10081 caaaagacat cgatgaaatt ttgatcacag aagaagaact taaggaaaag ataaaagagc 10141 ttgggaggca aatcacaaaa gactacaaag ggaaaaattt gatgttggta ggagttttaa 10201 aaggtgcttt aatgtttatg gctgatttgt caagacacat agatttgcct ttatcacttg 10261 attttatggc tgtttccagc tatggaagct caactcattc atcaggaata gtaaagataa 10321 tcaaagatct tgatataagc atagaaggca aagatgttct gattgtggaa gacataattg 10381 acagcggttt gactttgtct tacttaaggg aaactttact tggaaggaag ccaaaaagcc 10441 tgaaaatatg cacaatatta gacaaaccgg agagaagaga agcatctgta aaagtcgatt 10501 atgtaggatt taagatacct gataagtttg tcgtgggtta tggattggac tttgatgaaa 10561 agtacaggaa ccttcctttt ataggcgttt tgaaacctga aatgtacagc taagcccggg 10621 cctcgagaaa acaaaaggct cagtcggaag actgggcctt ttgttttggt acccctggga 10681 gaagttgcaa agctgcttta cctggaagac gaagagctgt tggaagaact ctatgatgtg 10741 gccaaatata taaaaaacaa aatatacgga aaaagagtgg ttttgtttgc tcctttatat 10801 acaagcaatg agtgtacaaa caactgcctt tactgcggtt tcaggcatga caacaaagag 10861 cttcacagaa agactttaag tcttgaagaa atagtggaag aagcaaaagc tattgaaaga 10921 cagggacata aaagattgct ccttatttgc ggagaagatc ccagaaagac taatgtaaag 10981 cattttaccg atgcaatgga ggcaatatat aaatccaccg atatcagaag aatcaatgtc 11041 gaggccgcgc cgatgacggt ggaagattac agggagctga agaaagccgg tataggaact 11101 tatgtgatat tccaggagac atatcacagg gaaacctata gaataatgca tcctgtgggc 11161 aaaaaagcga attatgactg gcgtataacg gcaatagaca gagcctttga aggcggtatt 11221 gacgatgtgg gtgtcggagc gctcttcgga ctttatgatt atagattcga agttttaggc 11281 cttttgatgc attgtatgca ctttgaagaa aaatatggtg tagggccgca caccatatct 11341 gttccgagac ttcgtcctgc cttgggagct cctttgaaag agattccgta caaagttacc 11401 gacaaggatt tcaagaaaat tgtggccata ttccgaattg ctgttccata tacgggaatt 11461 attctttcaa caagagaaag agctgaattc agggatgaac ttttaagtgt tggagtatct 11521 cagataagtg caggttccaa aaccaatccc ggaggatacc aggaggatga cgaccatgcg 11581 gatcagtttg aaataagtga caacaggagt ttgccgaaag taatggaaac aatatgtcaa 11641 caaggttata ttccaagctt ttgcaccgct tgctacagaa gatgtcgtac cggg //