Phylogeny of Intestinal Ciliates, Including Charonina ventriculi, and Comparison of Microscopy and 18S rRNA Gene Pyrosequencing for Rumen Ciliate Community Structure Analysis

Supplemental material

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  • Supplemental file 1 -

    Relative abundances of ciliate genera in fraction 1 of the rumen samples obtained from sheep S4 and cow C1 as observed by microscopic counts and 454-based pyrosequencing (Table S1), bright-field and single fluorescence in situ hybridization microscopic images (Fig. S1 and S2), randomized accelerated maximum likelihood tree (Fig. S3), and description of File S1.

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  • Supplemental file 2 -

    Accession numbers, taxonomic classifications, and 18S rRNA gene sequences of all 168 almost full-length (≥1,500 bp) intestinal ciliate 18S rRNA gene sequences used in this study. (File S1).

    XLSX, 102K