A novel role of farnesylation in targeting a mitotic checkpoint protein, human Spindly, to kinetochores

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• Video 3 -
  Mitotic timing of HeLa cells expressing GFP tubulin (green) and mCherry H2B (red) transfected with 100 nM control (scrambled) RNAi. Time-lapse imaging was performed using spinning disk confocal microscope (Axiovert 200M; Carl Zeiss) with a 40× objective. Images were captured using 100-ms exposure time for GFP and Cy3 every 5 min for 8 h. Representative video shows 6-h duration imaging. Bar, 24 µm.
• Video 2 -
  Mitotic timing of HeLa cells expressing GFP tubulin (green) and mCherry H2B (red) treated with 10 µM of FTI L-744832 for 24 h. Time-lapse was performed using spinning disk confocal microscope (Axiovert 200M; Carl Zeiss) with a 40× objective. Images were captured using 100-ms exposure time for GFP and Cy3 every 5 min for 18 h. Representative video shows 18-h duration imaging. Bar, 24 µm.
• Video 4 -
  Mitotic timing of HeLa cells expressing GFP tubulin (green) and mCherry H2B (red) transfected with 100 nM hSpindly RNAi and imaged after 33 h of transfection. Time-lapse imaging was performed using spinning disk confocal microscope (Axiovert 200M; Carl Zeiss) with a 40× objective. Images were captured using 100-ms exposure time for GFP and Cy3 every 5 min for 16 h. Representative video shows 15-h duration imaging. Bar, 24 µm.
• Video 1 -
  Mitotic timing of HeLa cells expressing GFP tubulin (green) and mCherry H2B (red) treated with solvent DMSO control for 24 h. Time-lapse imaging was performed using a spinning disk confocal microscope (Axiovert 200M; Carl Zeiss) with a 40× objective. Images were captured using 100-ms exposure time for GFP and Cy3 every 5 min for 8 h. Representative video shows 4-h duration of imaging. Bar, 24 µm