Published
March 30, 2015 //
JCB vol. 208 no. 7 881-896 The Rockefeller University Press, doi:
10.1083/jcb.201412085A novel role of farnesylation in targeting a mitotic checkpoint protein, human Spindly, to kinetochores
Files in this Data Supplement:
- Supplemental Material (PDF)
- Video 3 -
Mitotic timing of HeLa cells expressing GFP tubulin (green) and mCherry H2B (red) transfected with 100 nM control (scrambled) RNAi. Time-lapse imaging was performed using spinning disk confocal microscope (Axiovert 200M; Carl Zeiss) with a 40× objective. Images were captured using 100-ms exposure time for GFP and Cy3 every 5 min for 8 h. Representative video shows 6-h duration imaging. Bar, 24 µm. - Video 2 -
Mitotic timing of HeLa cells expressing GFP tubulin (green) and mCherry H2B (red) treated with 10 µM of FTI L-744832 for 24 h. Time-lapse was performed using spinning disk confocal microscope (Axiovert 200M; Carl Zeiss) with a 40× objective. Images were captured using 100-ms exposure time for GFP and Cy3 every 5 min for 18 h. Representative video shows 18-h duration imaging. Bar, 24 µm. - Video 4 -
Mitotic timing of HeLa cells expressing GFP tubulin (green) and mCherry H2B (red) transfected with 100 nM hSpindly RNAi and imaged after 33 h of transfection. Time-lapse imaging was performed using spinning disk confocal microscope (Axiovert 200M; Carl Zeiss) with a 40× objective. Images were captured using 100-ms exposure time for GFP and Cy3 every 5 min for 16 h. Representative video shows 15-h duration imaging. Bar, 24 µm. - Video 1 -
Mitotic timing of HeLa cells expressing GFP tubulin (green) and mCherry H2B (red) treated with solvent DMSO control for 24 h. Time-lapse imaging was performed using a spinning disk confocal microscope (Axiovert 200M; Carl Zeiss) with a 40× objective. Images were captured using 100-ms exposure time for GFP and Cy3 every 5 min for 8 h. Representative video shows 4-h duration of imaging. Bar, 24 µm