Table 2. Single-cell multiplex RT-PCR results
|
Sample (n)* transcripts |
FIA (12) |
FIN (9) |
Bath (6) |
FIA with RNase (1) |
FIN with RNase (1) |
FIA w/o RT (1) |
FIN w/o RT (1) |
|
GAPDH |
12 |
9 |
0 |
0 |
0 |
0 |
0 |
|
GFAP |
12 |
0 |
0 |
0 |
0 |
0 |
0 |
|
CaMKII |
0 |
9 |
0 |
0 |
0 |
0 |
0 |
|
Synaptophysin |
0 |
9 |
0 |
0 |
0 |
0 |
0 |
|
Syt I |
0 |
9 |
0 |
0 |
0 |
0 |
0 |
|
Syt II |
0 |
9 |
0 |
0 |
0 |
0 |
0 |
|
Syt IV |
12 |
0 |
0 |
0 |
0 |
0 |
0 |
|
Syt V |
5 |
8 |
0 |
0 |
0 |
0 |
0 |
*
Values in parentheses are the total no. of cells subjected to single-cell multiplex RT-PCR. FIA, freshly isolated astrocyte; FIN, freshly isolated neuron; Bath, saline adjacent to cells; RNase, DNase-free RNase; RT, reverse transcription.