Published April 13, 2015 // JCB vol. 209 no. 1 85-95
The Rockefeller University Press, doi: 10.1083/jcb.201409064

Sac2/INPP5F is an inositol 4-phosphatase that functions in the endocytic pathway

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    GFP-Sac2 dynamics at the late-stage clathrin-coated pits. COS7 cells were transfected with GFP-Sac2 and CLC-mRFP. Live imaging by spinning-disk confocal microscopy (UltraView VoX system; PerkinElmer) demonstrates endocytic clathrin-coated pit dynamics showing recruitment of GFP-Sac2 (green) at clathrin-coated pits just when the clathrin signal (CLC-mRFP, red) is about to disappear. Bar, 2 µm.