A role for disulfide bonding in keratin intermediate filament organization and dynamics in skin keratinocytes

Files in this Data Supplement:

• Supplemental Material (PDF)
• Video 5 -
  GFP-K14CF-C367–transfected K14^−/− mouse keratinocytes in primary culture showing a lack of stable perinuclear-concentrated networks. K14^−/− mouse keratinocytes were transfected with GFP-K14CF-C367 (green) and H2B-mCherry (red). Images were recorded using a laser scanning confocal microscope (LSM780-FCS; Carl Zeiss). Recording intervals were 5 min and the whole recording time was 6 h.
• Video 4 -
  GFP-K14CF-C367–transfected K14^−/− mouse keratinocytes in primary culture showing perinuclear-concentrated networks. K14^−/− mouse keratinocytes were transfected with GFP-K14CF-C367 (green) and H2B-mCherry (red). Images were recorded using a laser scanning confocal microscope (LSM780-FCS; Carl Zeiss). Recording intervals were 5 min and the whole recording time was 6 h.
• Video 1 -
  GFP-K14WT-transfected K14^−/− mouse keratinocytes in primary culture. K14^−/− mouse keratinocytes were transfected with GFP-K14WT (green) and H2B-mCherry (red). Images were recorded using a laser scanning confocal microscope (LSM780-FCS, Carl Zeiss). Recording intervals were 5 min and the whole recording time was 6 h.
• Video 3 -
  GFP-K14CF–transfected K14^−/− mouse keratinocytes in primary culture showing a motile behavior. K14^−/− mouse keratinocytes were transfected with GFP-K14CF (green) and H2B-mCherry (red). Images were recorded using a laser scanning confocal microscope (LSM780-FCS; Carl Zeiss). Recording intervals were 5 min and the whole recording time was 6 h.
• Video 2 -
  GFP-K14CF-transfected K14^−/− mouse keratinocytes in primary culture showing a stationary behavior. K14^−/− mouse keratinocytes were transfected with GFP-K14CF (green) and H2B-mCherry (red). Images were recorded using a laser scanning confocal microscope (LSM780-FCS; Carl Zeiss). Recording intervals were 5 min and the whole recording time was 6 h.