Evidence that Biosynthesis of the Second and Third Sugars of the Archaellin Tetrasaccharide in the Archaeon Methanococcus maripaludis Occurs by the Same Pathway Used by Pseudomonas aeruginosa To Make a Di-N-Acetylated Sugar

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 -

    Fig. S1, confirmation of in-frame deletions of targeted genes

    Fig. S2, mmp0350-mmp0354 and mmp0355-mmp0359 are each transcribed as an operon

    Fig. S3, transcript detection by RT-PCR

    PDF, 189K

  • Supplemental file 2 -

    Table SA1, strains and plasmids

    Table SA2, primers used to construct in-frame deletions

    Table SA3, primers used to construct gene complements

    Table SA4, primers used to screen and confirm deletion mutants

    Table SA5, primers used for RT-PCR

    PDF, 250K