Symbiodinium superoxide dimutase (Mn/Fe) amino acid alignment ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 5 15 25 35 45 55 65 75 85 95 105 115 125 135 145 155 165 175 185 195 B1 (Ap1) SymMnSOD1 KJ672521 MAPRRFRGAA ALVVGAMLVC ----CLERGF VSPTNAT--- RRNLAAGFAS GLAGVLGLES AQAYDLPDLP YAYDALEPSI DKATMEFHHD KHHLTYVTNI NKAMEGK--- ----TQPPLV ELQKTAIKDG --AAFRNSGG GAYNHNFFWK EMAPTGKGGK PSAKLASAID ESFGSMDDFK AKFQAAGAPG ARFGSGWVWL B1 (Mf1.05b) SymMnSOD1 Assembl MAPRRFRGAA ALVVGAMLVC ----CLERGF VSPTNAT--- RRNLAAGFXS GLAGVLGLES AQAYDLPDLP YAYDALEPSI DKATMEFHHD KHHLTYVTNI NKAMEGK--- ----TQPPLV ELQKTAIKDG --AAFRNSGG GAYNHNFFWE EMAPTGKGGK PSAKLASAID ESFGSMDDFK AKFQAAGAPG ARFGSGWVWL C1 (CCMP2466) SymMnSOD1 AHI543 MA-RR-RGAV ALVLGCLLVC ----CLERGF VSPTNAT--- RRSLAAGFAS GLAGVLGLES AQAYDLPDLP YAYDALEPSI DKATMEFHHD KHHLTYVTDI NKAMEGM--- ----TQPPLV ELQKTAIKDG --AAFRNSGG GAYNHNFFWL EMAPTGKGGK PSEKLAKAID DSLGSMDDFK AKFEAAGAPG ARFGSGWVWL C3 (Mp) SymMnSOD1 KJ672520 MAPRRFRGAA ALVVGCLLVC ----CLERGF VSPTNAT--- RRSLAAGFAS GLAGVLGLES AQAYDLPDLP YAYDALEPSI DKATMEFHHD KHHLTYVTNI NKAMEGK--- ----TQPPLV ELQKTAIKDG --AAFRNSGG GAYNHNFFWL EMAPTGKGGK PSEKLAKAID DSFGSMDDFK TKFEAAGAPG ARFGSGWVWL C3 (A.aspera) SymMnSOD1 FE8660 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ------DDFK AKFQAAGAPG ARFGSGWVWL C15 (M.digitata) SymMnSOD1 KJ6 MAPRRFRGAA ALVVGCLLVC ----CLDCGF VSPTNAT--- RRSLAAGFAS GLAGVLGLES AQAYDLPDLP YAYDALEPSI DKATMEFHHD KHHLTYVTNI NKAMEGK--- ----TQPPLV ELQKSAIKDG --AAFRNSGG GAYNHNFFWL EMAPTGKGGK PSEKLAKAID DSFGSMDDFK AKFEAAGAPG ARFGSGWVWL B1 (Mf1.05b) SymMnSOD2 Assembl MAPRRFRGAA ALVVGAMLAC ----CLERGF VSPTNAT--- RRNLAAGFAS GLAGVLGLES AQAYDLPDLP YAYDALEPSI DKATMEFHHD KHHLTYVTNI NKAMEGK--- ----TQPPLV ELQKTAIKDG --AAFRNSGG GAYNHNFFWL EMAPTGKGGK PSEKLASAID ESFGSMDDFK AKFEAAGAPG ARFGSGWVWL B1 (Mf1.05b) SymMnSOD2 rep_c13 MAPRRFRSAA ALVVGAMLVC ----CLERGF VSPTNAT--- RRNLAAGFAS GLAGVLGLES AQAYDLPDLP YAYDALEPSI DKATMEFHHD KHHLTYVTNI NKAMEGK--- ----TQPPLV ELQKTAIKDG --AAFRNSGG GAYNHNFFWL EMAPTGKGGK PSEKLASAID ESFGSMDDFK AKFEAAGAPG ARFGSGWVWL D (A.hyacinthus) SymMnSOD2 GAF MA-RRFRG-A ALLVAAMLLC ----CWHCSF VSPQGAS--- RREMAAGLAT GLVGLLS-ES ARAYDLPDLP YAYDALEPSI DKATMEFHHD KHHLTYVTNI NKAMEGK--- ----SQPPLV ELQKSAIKDG --AAFRNSGG GAYNHNFFWL EMAPTGKGGK PSEKLSKAID DSFGSMDDFK AKFEAAGAPG ARFGSGWVWL F1 (CCMP2468) SymMnSOD2 Assemb MAPRRCRGAA ALVVGCLLVC ----CLERGF VSPSNAS--- RRTLAAGFAS GLAGVLGLES AQAYDLPDLP YAYDALEPSI DKATMEFHHD KHHLTYVTNI NKAMEGK--- ----TQPPLV ELQKSAIKDG --AAFRNSGG GAYNHNFFWL EMAPTGKGGK PSDKLAKAID ESFGSMDDFK AKFEAAGAPG ARFGSGWVWL A1 (Casskb8) SymMnSOD3 rep_c41 ---------- ---------- ---------- ---------- MLGLRCWLVS --LVASGVHA GDVFTLPALP YEYDALEPHI DAETMKIHHG KHHAAYVAGL NAAISKS--- TTDGSMTALT SLQPNASKTG --AAHRNHGG GHYNHALFWV NLAPASGSSE PSPDLAAAID DKFGSLDGLK QAFSKAAMG- -RFGSGWAWL A1 (CCMP2467) SymMnSOD3 Assemb ---------- ---------- ---------- ---------- MLGLRCWLVS --LVASGVHA GDVFTLPALP YEYDALEPHI DAETMKIHHG KHHAAYVAGL NAAISKS--- TTDG------ ---------- --AAHRNHGG GHYNHALFWV NLAPASGSSE PSPDLAAAID DKFGSLDGLK QAFSKAAMG- -RFGSGWAWL A1 (CCMP2467) SymMnSOD3 Assemb ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- B1 (Mf1.05b) SymMnSOD3 _c19510 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- D (A.hyacinthus) SymMnSOD3 GAF ---------- ---------- ---------- ---------M WFHVPTSAMA --MALWVGAA GEIFSLPALP YEYDALEPYI DEQTMRIHHG KHHATYVAGL NAELQGK--- KVSGDMVALA ELQRTAPATS --PATRNHGG GHYNHALFWV NLASASGSSE PSPELGAAIE DRFGGLDGLK EAFNKIALS- -RFGSGWAWL A1 (Casskb8) MnSOD rep_c710 MA-RRVRGVS ALVVAALVLV AARQCMDLCF VTPGAGSS-T RRGVATTVAT GLASLIGLES AKAYDLPDLP YAYDALEPSI DKATMEFHHD KHHLTYVTNI NKALKDK--- ----KQPPLV ELQKTAIKDG --AAFRNSGG GAYNHNFFWL EMAPTGTGGS PSEKLSSAID ASFGSMDDFK AKFEAAGAPG ARFGSGWVWL A1 (CCMP2467) MnSOD Assembly4 MA-RRVRGVS ALVVAALVLV AARQCMDLCF VTPGAGSS-T RRGVATTVAT GLASLIGLES AKAYDLPDLP YAYDALEPSI DKATMEFHHD KHHLTYVTNI NKALKDK--- ----KQPPLV ELQKTAIKDG --AAFRNSGG GAYNHNFFWL EMAPTGTGGS PSEKLSSAID ASFGSMDDFK AKFEAAGAPG ARFGSGWVWL A1 (CCMP2467) MnSOD Assembly3 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- A1 (Casskb8) MnSOD _c18645 MA-RRVRGIL ALAVAAALVW ---QCVDLAF LTPAPESAPT RRHLAAGLAS GLAGLVGLES AQAYDLPDLP YAYDALEPSI DKATMEFHHD KHHLTYVTNI NKALEGK--- ----TQPPLV ELQKTAIKDG --AAFRNSGG GAYNHNFFWL EMAPTGKGGA PSDKLAKAID ESFGSLDDFK AAFEAAGAPG ARFGSGWVWL A1 (CCMP2467) MnSOD Assembly1 MA-RRVRGIL ALAVAAALVW ---QCVDLAF LTPAPESAPT RRHLAAGLAS GLAGLVGLES AQAYDLPDLP YAYDALEPSI DKATMEFHHD KHHLTYVTNI NKALEGK--- ----TQPPLV ELQKTAIKDG --AAFRNSGG GAYNHNFFWL EMAPTGKGGA PSDKLAKAID ESFGSLDDFK AAFEAAGAPG ARFGSGWVWL B1 (Mf1.05b) MnSOD _c29099 ---------- ---------- ---------- ---------- MR--VVWLVA --MVQ--LVK GDIFSLPALP YEYDALEPYI DEQTMRIHHE KHHATYVAGL NAALAAKGGA PAKGDVSFLT DLQREAAKTS --PAMRNHGG GHYNHALFWV NLASASSPSE PSADLGAAID DRFGSLDGLK GAFSKVALS- -RFGSGWA-- A (PF-2005) SymFeSOD AY916504 ---------- ---------- ---------- ---------- ---------- ---------- -MAFDLPELP YGRAGLAPHI SEETLNFHHG KHHNAYVTNL NKLIDG---- -TDLAGASLE AIVKAAAGDA SKAGLFNNAA QVWNHTFYWH SMKP-GGGGA PHGKIAEMID RDFGSYDEFA TQFKAAG--G GQFGSGWAWL B1 (Ap1) SymFeSOD KJ672519 ---------- ---------- ---------- ---------- ---------- ---------- ---------- YGRDGLAPHI SEETLNFHHG KHHNAYVTNL NKMIDG---- -TDLAGASLE EIVNAAAGDA SKAGLFNNAA QVWNHTFYWH SMKP-GGGGA PHGKIAELID RDFGSYDEFA TQFKAAG--G GQFGSGWAWL E (CCMP421) SymFeSOD KJ672517 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- -----NFHHG KHHNAYVTNL NKLIDG---- -TDLADASLE DIVKAAAGDA AKAGLFNNAA QVWNHTFYWH SMKP-GGGGA PHGKIAELIE RDFGSYDEFA TQFKAAG--G GQFGSGWAWL F1 (Mv) SymFeSOD KJ672518 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- --ETLNFHHG KHHNAYVTNL NKLIDG---- -TDLADASLE DIVKAAAGDA AKAGLFNNAA QVWNHTFYWH SMKP-GGGGA PHGKIAELIE RDFGSYDEFA TQFKAAG--G GQFGSGWAWL ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ... 205 215 225 235 245 255 265 275 285 295 305 315 B1 (Ap1) SymMnSOD1 KJ672521 VVDGNKKLAI TSTPNQDNPL MEGVDG--TT GIPILGCDVW EHAYYLKYQY RRPDYIKAWW D-VVNWDQVS AWYDGAMSGK ---------- ---------- ---------- ---------- --- B1 (Mf1.05b) SymMnSOD1 Assembl VVDGNKKLAI TSTPNQDNPL MEGVDG--TT GIPILGCDVW EHAYYLKYQY RRPDYIKAWW D-VVNWDQVS AWYDGAMSGK APAA*----- ---------- ---------- ---------- --- C1 (CCMP2466) SymMnSOD1 AHI543 VVDGDKKLAI TSTPNQDNPL MEGVEG--TA GIPILGCDVW EHAYYLKYQY RRPDYIKAWW D-VVNWDQVS AWYEDALGGK APAA*----- ---------- ---------- ---------- --- C3 (Mp) SymMnSOD1 KJ672520 VVDGDKKLAI TSTPNQDNPL MEGVEG--TA GIPILGCDVW EHAYYLKYQY RRPDYIKAWW D-VVNWDQVS KWYNDALGGK APAA*----- ---------- ---------- ---------- --- C3 (A.aspera) SymMnSOD1 FE8660 VVDGDKKLAI TSTPNQDNPL MEGVEG--TA GIPILGCDVW EHAYYLKYQY RRPDYIKAWW D-VVNWDQVS KWYNDALGGK APAA*----- ---------- ---------- ---------- --- C15 (M.digitata) SymMnSOD1 KJ6 VVDGDKKLAI TSTPNQDNPL MDGVEG--TA GIPILGCDVW EHAYYLKYQY RRPDYIKAWW D-VVNWDQVS AWYEDALGGK APAA*----- ---------- ---------- ---------- --- B1 (Mf1.05b) SymMnSOD2 Assembl VVDGDKKLAI TSTPNQDNPL MEGVDG--TA GIPILGCDVW EHAYYLKYQY RRPDYIKAWW D-VVNWDQVS AWYEDALGGT APTADSATSA AALSGRKTA- ---ATSSFSL KLGFEANARY GS* B1 (Mf1.05b) SymMnSOD2 rep_c13 VVDGDKKLAI TSTPNQDNPL MEGVDG--TA GIPILGCDVW EHAYYLKYQY RRPDYIKAWW D-VVNWDQVS AWYEDALGGT APTADSATSA AALSGRKASP SKPATSSFSL KLGFEANARY GS* D (A.hyacinthus) SymMnSOD2 GAF VVTDDKKLAI TSTPNQDNPL MDGVEG--TA GIPILGCDVW EHAYYLKYQY RRPDYIKAWW D-VVNWDQVS AWYEDALGGK APTADSATSA AALSGRKTS- -----SSFNL KLGFEANARY GS* F1 (CCMP2468) SymMnSOD2 Assemb VVDGDKKLAI TSTPNQDNPL MEGVEG--TA GIPILGCDVW EHAYYLKYQY RRPDYIKAWW D-VVNWDQVS AWYEDALGGK APSADSATSA AALSGRKTP- -----SSFSL KLGFEANARY GS* A1 (Casskb8) SymMnSOD3 rep_c41 GVTPQGSLAV TSTANQDNPL MEGLEYSVEK LIPILGLDVW EHAYYLKYQN RRADYVSAFW N-VVNWKKVS RNYEEYAKKG QPVPPTPTGG GHAVEL*--- ---------- ---------- --- A1 (CCMP2467) SymMnSOD3 Assemb GVTPQGSLAV TSTANQ---- ---------- ---------- ---------- -----VSAFW N-VVNWKKVS RNYEEYAK-- ---------- ---------- ---------- ---------- --- A1 (CCMP2467) SymMnSOD3 Assemb ---------V TSTANQDNPL MEGLEYSVEK LIPILGLDVW EHAYYLKYQN RRADYVSAFW N-VVNWKKVS RNYEEYAKKG QPVPPTPTGG GHAVEL*--- ---------- ---------- --- B1 (Mf1.05b) SymMnSOD3 _c19510 ---------V TSTANQDNPL MEGLEYSVEK LIPILGLDVW EHAYYLKYQN RRADYVSAFW N-VVNWKKVS RNYEEYAKKG QPVPPTPTGG GHAVEL*--- ---------- ---------- --- D (A.hyacinthus) SymMnSOD3 GAF GVAPDGQLKV TSTANQDNPL MEGLEYKVEK MIPILGLDVW EHAYYLKYQN RRADYVNAFW N-IINWKKVS RNYEEYAKKG KPVPPTPTGS GHAVEL*--- ---------- ---------- --- A1 (Casskb8) MnSOD rep_c710 VVTADKKLAI TSTPNQDNPL MEGVEG--TP GIPILGCDVW EHAYYLKYQY RRPDYIKAWW N-VVNWKQVS AWYEGAMGGS APTA*----- ---------- ---------- ---------- --- A1 (CCMP2467) MnSOD Assembly4 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- --- A1 (CCMP2467) MnSOD Assembly3 -VTADKKLAI TSTPNQDNPL MEGVEG--TP GIPILGCDVW EHAYYLKYQY RRPDYIKAWW N-VVNWKQVS AWYEGAMGGS APTA*----- ---------- ---------- ---------- --- A1 (Casskb8) MnSOD _c18645 VVTDDKKLAI TSTPNQDNPL MDGVDG--TA GIPILGCDVW EHAYYLKYQY RRPDYIKAWW D-VVNWNQVS AWYEDALGGT APSADSATAT AALAGQR--- ---------- ---------- --- A1 (CCMP2467) MnSOD Assembly1 VVTDDK---- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- --- B1 (Mf1.05b) MnSOD _c29099 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- --- A (PF-2005) SymFeSOD AY916504 ILK-DGKLAI TKTPNAETPL ---TEDGAT- --PLLTMDVW EHAYYLDYQN SRPNYMASFL DNLVNWDFAN QNLADAS*-- ---------- ---------- ---------- ---------- --- B1 (Ap1) SymFeSOD KJ672519 VLK-DGKLAI TKTPNAETPL ---TEAGVT- --P------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- --- E (CCMP421) SymFeSOD KJ672517 VLK-DGKLAI TKTPNAETPL ---TEAGVT- --PILTMDVW EHAYYLDYQ- ---------- ---------- ---------- ---------- ---------- ---------- ---------- --- F1 (Mv) SymFeSOD KJ672518 VLK-DGKLAI TKTPNAETPL ---TEA---- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---