Symbiodinium ascorbate peroxidase amino acid alignment ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 5 15 25 35 45 55 65 75 85 95 105 115 125 135 145 155 165 175 185 195 A1 (Casskb8) SymAPX1 rep_c1302 ---------M GNTQCCQSSD TS--PDAAAE AMPAMESWPA NPGKS-ALDV PTVSGGNTAQ ERTYKVRLVK KEGQKLGLDV DYMAERSVLP ILVISGGIAE MWNKQHPDKK MNTGDSITEV NGIKGNVALM LEKCKADQTL ELTVVKCLTY GHLVADLEKL MLVKGCGPIM VRLSWHDAGV FNG----ADG CPNAAMRLAG A1 (CCMP2467) SymAPX1 GAKY0104 ---------M GNTQCCQSSD TS--PDAAAE AMPAMESWPA NPGKS-ALDV PTVSGGNTAQ ERTYKVRLVK KEGQKLGLDV DYMAERSVLP ILVISGGIAE MWNKQHPDKK MNTGDSITEV NGIKGNVALM LEKCKADQTL ELTVVKCLTY GH-------- ---------- ---------- ---------- ---------- A1 (Casskb8) SymAPX1 rep_c1797 ---------M GNASCCKS-D TAPGVEATVD AKPALDSSLN A--------L PAVTGGKTDK ERHYSIKLVK SGGLKLGLDV DYMAERSVLP IMSVTGGVAE KWNQDNPEMQ IRKGDSIVEV NGTTGDVAQM LDKCKTDVEL ELTLCRCLNY DHLVADLEKL ISAKNCGPIM IRLSWHDAGV FNG----ADG CPNAAMRLAG A1 (CCMP2467) SymAPX1 Locus_98 ---------M GNASCCKS-D TAPGVEATVD AKPALDSSLN A--------L PAVTGGKTDK ERHYSIKLVK SGGLKLGLDV DYMAERSVLP IMSVTGGVAE KWNQDNPEMQ IRKGDSIVEV NGTTGDVAQM LDKCKTDVEL ELTLCRCLNY DHLVADLEKL ISAKNCGPIM IRLSWHDAGV FNG----ADG CPNAAMRLAG A1 (Casskb8) SymAPX1 rep_c1337 ---------M GNTACCQSEA NDGVADAHVD AKPILESALP A--------A PFISGGDSEQ ERIYQVTLSK SPGQKLGLDV DYMAERKILP IMHVTGGIAE EWNKKYPDRK LSSGDSILEV NGISGDVVEM LERCKADDTL KMKLCRCLTY DHLVEDLEKL IRRKDCGPIM VRLSWHDAGI YNG----ADG CPNAAMRLPG A1 (CCMP2467) SymAPX1 Locus_51 ---------M GNTACCQSEA NDGVADAHVD AKPILESALP A--------A PFISGGDSEQ ERIYQVTLSK SPGQKLGLDV DYMAERKILP IMHVTGGIAE EWNKKYPDRK LSSGDSILEV NGISGDVVEM LERCKADDTL KMKLCRCLTY DHLVEDLEKL IRRKDCGPIM VRLSWHDAGI YNG----ADG CPNAAMRLPG A1 (CCMP2467) SymAPX1 Assembly ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- -RLSWHDAGV FNG----ADG CPNAAMRLAG B1 (Mf1.05b) SymAPX1 Assembly1 ---------M XNSQCCKSVD TSP--DEA-D AMPAMESWPA ASNVPNGLQV PVVSGGSSVQ ERMYKVTLMK AEGQKLGLDV DYMAERSVLP VLEIKGGIAE QWNKQNPSKR INQGDSVVEV NGVRGNVALM LEKCKADPTL ELTICKCLTF GHLVQDLRKL IGIKGCGPIL IRLSWHDAGV FNG----SDG CPNAAMRLPG B1 (Mf1.05b) SymAPX1 rep_c960 ---------- ---------- --PSSDATVD ARPAMDSSLQ V--------L PPVAGGNTEK ERVYSVKLTK SGDRKLGLDV DFMAERVVLP IMTVTGGVAE VWNQEHPELP ISKGDSILEV NGVSGDVAAM LDRCKTDMDL DMTLCKCLNY DHLVADLEKL VSNKGCGPIM IRLSWHDAGV YNG----VDG CPNAAMHLKG B1 (Ap1) SymAPX1 KJ672513 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- -YMAERSVLP ILVISGGLAE QWNKEHPERK MNAGDGVVEV NGVRGNVALM LEKCKSDPTL ELTICKCLTY NHLVADLEKL ISIKGCGPIM IRLSWHDAGV FNG----LDG CPNAAMRLPG B1 (Mf1.05b) SymAPX1 Assembly3 ---------- ---------- ---------- ---FMETWPA -PGKP-PMEV PAVSGG-GPQ ERWYKVTLVK SAGMKLGMDV DYMAERSVLP ILVISGGLAE QWNKEHPERK MNAGDGVVEV NGVRGNVALM LEKCKSDPTL ELTICKCLTY NHLVADLEKL ISIKGCGPIM IRLSWHDAGV FNG----LDG CPNAAMRLPG B1 (Mf1.05b) SymAPX1 rep_c3008 ---------- ---------- ---------- ---------- ---------- ---SGGNTVQ ERMYKVSLTK VDGVSLGMDV DYLAERCVLP ILFIAGGLTE QWNKEHPENK ISTGDSIVEV NGVRGDVANM LQKCKVDPVL DLTICKCLTY GHLVQDLENL ISIKGCGPIM IRLSWHDAGV FNG----QNG CPNAAMRLAG B1 (Mf1.05b) SymAPX1 Assembly4 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---TGNVTEI LEKCKADSTL KMKICRCLTY EFLLEDLEKL IRHKNCGPIL IRLSWHDAGV FNG----VDG CPNXAMRLKG C1 (CCMP2466) SymAPX1 KF835562 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- --------EV NGVRGNVALM LEKCKHDPTL ELTICKCLTY GHLISDLQKL MTIKGCGPIM IRLSWHDAGV FNG----VDG CPNAAMRLAG C3 (S.hystrix) SymAPX1 HM15669 ---------M G-AQCCKSVD TG--PDGMGE AMPTMESWPP SG---SPMEL PAVSGG-RVE ERMYKVTLVK KEGQKLGLDV DFMAERSVLP ILTISGGIAE QWNNQHPYRK MSTSDSIVEV NGVRGNVALM LEKCKHDPTL ELTICKCLTY GHLISDLQKL MTIKGCGPIM IRLSWHDAGV FNG----VDG CPNAAMRLAG C3 (A.aspera) SymAPX1 FE865713 ---------M G-AQCCKSVD TG--PDGMGE AMP-MESWPP SG---SPMEL PAVSGG-RVE ERMYKVTLVK KEGQKLGLDV DFMAERSVLP ILTISGGIAE QWNNQHPYRK MSTSDSIVEV NGVRGNVALM LEKCKHDPTL EL-------- ---------- ---------- ---------- ---------- ---------- C15 (M.digitata) SymAPX1 KJ672 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- -------VEV NGVRGNVALM LEKCKHDPTL ELTICKCLTY GHLISDLQKL MTTKGCGPMM IRLSFNDAGM FNG----VDG CPNAAMRLAG D (A.hyacinthus) SymAPX1 GAFP0 ---------M GNTQCCKSVD TS--PDAAAE AMPTLESWPA NPGKP-LIEV PAVSGGNSPQ ERMYKVSLVK TEGQKLGLDV DYMAERSVLP ILVISGGIAE QWNRSHPERK MNTGDSIVEV NGIRGNVALM LEKCKVDATL ELTICKCLTY GHLVADLEKL ISIKGCGPIM VRLSWHDAGV FNG----VDG CPNAAMRLAG D (A.hyacinthus) SymAPX1 GAFP0 ---------M G-ATCCKT-D TAPSSDATVE ARPALDSSLA ---------L PLVTGGTTEK ERLYTVKLTK SGDRKLGLDV DFMAERKVLP IMTVTGGVAE IWNQEHPELQ ISKGDSILKV NGVTGDVAAM LDRCKTDMEL ELTLCKCLNF EYLVVDLEKL ITSKGCGPLM LRLSWHHKCI FYA----GEK PH-------- F1 (Mv) SymAPX1 KJ672514 ---------M G-VQCCKSAD TS--PDAAG- DMPMMESWPP TK-----MDL PALSGG-KAD ERMYKVTLVK QAGKKLGLDV DYMVERSVLP VLGISGGTAE EWNEKNPNKK MNIGDSLVEV NGVRGNVALM LEKCKNDATL ELTLCKSLTF GHLVSDLEKL VSLKGCGPIL IRLSWNDASV FNG----ENG CPNAAMRLSG F1 (CCMP2468) SymAPX1 Assembly ---------M G-VQCCKSAD TS--PDAAG- DMPMMESWPP TK-----MDL PALSGG-KAD ERMYKVTLVK QAGKKLGLDV DYMVERSVLP VLGISGGTAE EWNEKNPNKK MNIGDSLVEV NGVRGNVALM LEKCKNDATL ELTLCKSLTF GHLVSDLEKL VSLKGCGPIL IRLSWNDASV FNG----ENG CPNAAMRLSG F1 (CCMP2468) SymAPX1 Assembly ---------M G-ASCCTPSE TASGSDATVD ARPVLDSSLQ T--------W PPVAGGNTEK ERIFIVKLRK TGELKLGLDV DFMAERSVLP IMTVTGGVAE KWNQEHPELP ISRGDSILEV NGVSGDLTQM LDRCKTDTEL QLTLCKCLSY DHLVSDLEKL ISSKGCGPIL VRLSWQDAGV FNG----VDG CPNAAMRLLG F1 (CCMP2468) SymAPX1 Assembly ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- A1 (Casskb8) SymAPX2 Assembly1 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- -PSSGGVAGV TXLVGAGLVA SAALXRSVKP RGGKKNLVSM AARGGE---- --------SY DALVKDLETL VKEKECGPIL VRLSWHDAGV FSD-GKLKGG CPNAAMRFTD A1 (CCMP2467) SymAPX2 Locus_19 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------P RGGKKNLVSI AARGGE---- --------SY DALVKDLETL VKEKECGPIL VRLSWHDAGV FSD-GKLKGG CPNARMRFTD A1 (CCMP2467) SymAPX2 KJ672516 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- -------VSM AARGGE---- --------SY DALVKDLETL VKEKECGPIL VRLSWHDAGV FSD-GKLKGG CPNAAMRFTD B1 (Mf1.05b) SymAPX2 rep_c2343 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- C3 (A.aspera) SymAPX2 Assembly ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- D (A.hyacinthus) SymAPX2 GAFP0 ---------- ---------- ---------- ---------M TKRGNSKVAQ AVALAATATA AGSAFSMMAA PSALESRITS TPSQIGATRS TTGTYGVAGV SSVVGAGLVA SAAFGRRSAK QSRQRGVVSM AARGGE---- --------SY DSLVKDLQEL VKTKQCGPIL IRLSWHDAGV FSD-GKLKGG CPNAAMRFTD D (A.hyacinthus) SymAPX2 GAFP0 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- --------ML LASPSFFGRF AWLLSLVSLA WPALG-DAAT EGQCDAATGK CGSMAE---- --------SY DALVKDLEEL VRTKHCGPIL IRLSWHDAGV FSN-GKLKGG YSNAAMRFTD A1 (Casskb8) SymAPX3 Assembly2 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- --------MG NACSDGCFGM LGSK-KVSQL KACKQDLKKF IDSKNCHPIM VRLAWHDSGT YDQRIKDFPE RGGANGAIRF A1 (Casskb8) SymAPX3 Assembly3 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- --------MG NACSDGCFGM LGSK-KVSQL RACKQDLKKF IDGKNCHPIM VRLAWHDSGT YDQRIKNFPE RGGANGAIRF A1 (Casskb8) SymAPX3 rep_c280 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- --------MG NACSDGCFGM LASK-KVSQL RACKQDLKKF IDSKNCHPIM VRLAWHDSGT YDQRIKNFPE RGGANGAIRF A1 (CCMP2467) SymAPX3 Locus_31 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- A1 (CCMP2467) SymAPX3 Assembly ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- --------MG NACSDGCFGM LASK-KVSQL RACKQDLKKV IDSKNCHPIM VRLAWHDSGT YDQRIKDFPE RGGANGAIRF D (A.hyacinthus) SymAPX3 GAFP0 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- --------MG NACSNGCFAM LGGNPKAAEL RACKEEIKTL IETRNCHPIL VRLAWHDSGT YDQRIKDWPQ RGGANGAIRF B1 (Mf1.05b) SymAPX4 Assembly2 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- -----PLEEY VRCRAELLKF IDENNCAPIM VRLAWHDSGN FDHRITEFPD RGGANGSIIH D (A.hyacinthus) SymAPX4 GAFP0 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---MAQMEHY VKCREELLKF IDENNCAPIM VRLAWHDSGN FDKRISSWPE CGGANGSIIH F1 (CCMP2468) SymAPX4 KC944388 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- A1 (Casskb8) SymAPX5 rep_C1877 MAGKMPKVDD GGDGTNLGDE DLVCNCHSVP KKVIRDAIKN GAHTFEDIKK CTKAGTGCGT CIRTGPQPKL LSHTLNSLGV EKGCCKSLPF EADDIEDLAK ARQLKTYEEL VSNLGYPPAA VEGDKAALAG VLDRIGGKPK GEGMDHAGQL KALKEDLWKF VDKMNCNPIL LRLAWHDAGT YDKSKSDFGD RGGANGSIRF A1 (CCMP2467) SymAPX5 Locus_10 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- -------KPK GEGMDHAGQL KALKEDLWKF VDKMNCNPIL LRLAWHDAGT YDKSKSDFGD RGGANGSIRF D (A.hyacinthus) SymAPX5 Assem MAGQMPKVDD GGDGTNLGDD DHVCNCHSVP KKVIRDAIMK GAHTFEEIKK CTKAGTGCGT CIRNGPQPKL LAHTLNAKGV EAGVCKSLPF THDDIEDLAK ARQLKSYDEL VSHLGYPSAA AESDKTALGG LLDRIGGKPK GEGLDHLGQL KALKEDLWKF VDKMNCNPIL VRLAWHDAGT YDKSKTAFGE RGGANGSIRF A1 (CCMP2467) SymAPX6 Locus_36 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------M GQRCHGVVAV ALCLCCLRGL TRCFLTSTPC PGLRRRDHTL HWLVATGFAP AAEAFEGDAA CIREAARTQT LQDPVLAPSL LRLAFHDAAT RRPGD---SR RQGPNGSIRF B1 (Mf1.05b) SymAPX6 rep_c6573 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- -----SAPSF LRLAFHDAAT KR------SD AQGPNGSIRF A1 (Casskb8) APX Assembly4 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- -MWRLATIFS QMRASFEAAP CAGEDLVSQL RACKEELKKF INEQNCAPIL IRLAWHDSGT YDQRIAEFPQ RGGANGAIRF A1 (CCMP2467) APX Locus_35678 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- -MWRLATIFS QMRASFEAAP CAGEDLVSQL RACKEELKKF INEQNCAPIL IRLAWHDSGT YDQRIAEFPQ RGGANGAIRF ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 205 215 225 235 245 255 265 275 285 295 305 315 325 335 345 355 365 375 385 395 A1 (Casskb8) SymAPX1 rep_c1302 GGEHAFGANA GLPQVAIPLL QAISDKYVPR LISHADLWAL AANVAIKVMG GPDIVTHFGR VDAQSPSDGA TSAAG----- --RLPDGDKD ---------- --AQHLRDIF RP-KGFTDKD IVALSGAHTV GACHADRS-- ---------- ---------- ---------- -GFEG--PWT ADKLKFDNTY FQDLLNKTWS A1 (CCMP2467) SymAPX1 GAKY0104 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- A1 (Casskb8) SymAPX1 rep_c1797 SGEHSLGANA GLPEVALALL GPITEKYVPR LISHADLWAL AANVSIKAMG GPEIPTRFGR LDAHHASDGV SSADG----- --RLPDGDKD ---------- --ANHIRGIF GP-KGFEDRD MVALSGAHTV GMCHGDRS-- ---------- ---------- ---------- -GFEG--PWT DDKLLFDNSY FKDLLQKPWT A1 (CCMP2467) SymAPX1 Locus_98 SGEHSLGANA GLPEVALALL GPITEKYVPR LISHADLWAL AANVSIKAMG GPEIPTRFGR LDAHHASDGV SSADG----- --RLPDGDKD ---------- --ANHIRGIF GP-KGFEDRD MVALSGAHTV GMCHGDRS-- ---------- ---------- ---------- -GFEG--PWT DDKLLFDNSY FKDLLQKPWT A1 (Casskb8) SymAPX1 rep_c1337 GGEHALAANA GLPQVAIPLL EPIAAKYVPR MISHADLWAL AANVAIKVMG GPELTTRFGR LDAERVGDGA SSATG----- --RLPDGDKD ---------- --ARHLRDIF YP-KGFDDKA IVALSGAHTV GSCHLDRS-- ---------- ---------- ---------- -GFDG--AWT ADKLKFDNSY FKDLMHRSWS A1 (CCMP2467) SymAPX1 Locus_51 GGEHALAANA GLPQVAIPLL EPIAAKYVPR MISHADLWAL AANVAIKVMG GPELTTRFGR LDAERVGDGA SSATG----- --RLPDGDKD ---------- --ARHLRDIF YP-KGFDDKA IVALSGAHTV GSCHLDRS-- ---------- ---------- ---------- -GFDG--AWT ADKLKFDNSY FKDLMHRSWS A1 (CCMP2467) SymAPX1 Assembly GGEHAFGANA GLPQVAIPLL QAISDKYVPR LISHADLWAL AANVAIKVMG GPDIVTHFGR VDAQSPSDGA TSAAG----- --RLPDGDKD ---------- --AQHLRDIF RP-KGFTDKD IVALSGAHTV GACHADRS-- ---------- ---------- ---------- -GFEG--PWT ADKLKFDNTY FQDLLNKTWS B1 (Mf1.05b) SymAPX1 Assembly1 GGEHAFAANS GLPQVAIPLL QAISEKYVPS LISHSDLWTL AANVAIEVMG GPEITTHFGR FDVKSHDQGV QSAQG----- --RLPDGDKD ---------- --AKHLRDIF YP-KGFSDKD IVALSGAHTV GACHPERS-- ---------- ---------- ---------- -GFEG--QWT EDKLKFDNSY FKDLLQKTWT B1 (Mf1.05b) SymAPX1 rep_c960 SGEHGMAANA GLPDVALGLL RSIADKYVPR LISNADLWAL AANVAIKMMG GPDVPTRFGR LDVTKTSEGA PDATG----- --RLPDGDKD ---------- --AQHLRDIF AP-KGFDDKE IVALSGAHTV GMCHIDRS-- ---------- ---------- ---------- -GFEG--PWT SNKLSFDNSY FKDMLNKTWV B1 (Ap1) SymAPX1 KJ672513 GGEYALGANA GLPQVAIPLL QAISEKYVPM LISHADLWAL AANVAIKVMG GPDIPTNFGR YDVPDKKEGA QSAAG----- --RLPDGDKD ---------- --AKHLRDIF YP-KGFTDKD IVALSGAHTV GACHAERS-- ---------- ---------- ---------- -GFEG--AWT DDKLKFDNSY FQDLLNKKWD B1 (Mf1.05b) SymAPX1 Assembly3 GGEYALGANA GLPQVAIPLL QAISEKYVPM LISHADLWAL AANVAIKVMG GPDIPTNFGR YDVPDKKEGA QSAAG----- --RLPDGDKD ---------- --AKHLRDIF YP-KGFTDKD IVALSGAHTV GACHAERS-- ---------- ---------- ---------- -GFEG--AWT DDKLKFDNSY FQDLLNKKWD B1 (Mf1.05b) SymAPX1 rep_c3008 GGEYAFGANA GLPQVAIPLL KDITDKYVPR LISHADLWAL AANVAIKVMG GPSIVTHFGR LDAQGHSEGA QEVDG----- --RLPDGDKD ---------- --AKHLRDIF WP-KGFTDQD IVALSGAHTV GACHGNRS-- ---------- ---------- ---------- -GFEG--PWT DEKLKFDNSY F--------- B1 (Mf1.05b) SymAPX1 Assembly4 TAEREMNANA GLPQIALNLL QPITAKYVPR LISHADLWVV AANVAIKVMG GPTITTRYGR TDASHGGESV SSASG----- --RLPEADKD ---------- --ASHLRSIF NP-KGFDDKE MVALSGAHTV GSCHLDRS-- ---------- ---------- ---------- -GYEG--DWT DQKLKFDNSY FKDLLNKQWQ C1 (CCMP2466) SymAPX1 KF835562 GGEHALGANA GLPQVAIPLL QAITEKYVPG LISHADLWAL AANVAIKVMG GPDIITHFGR FDCLTCNEGA QSAAG----- --RLPDGDKD ---------- --AQHLREIF CP-KGFTDKD IVALSGAHTV GACHADRS-- ---------- ---------- ---------- -GFEG--PWT DDKLKFDNSY FKDLLNKKWT C3 (S.hystrix) SymAPX1 HM15669 GGEHALGANA GLPQVAIPLL QAITEKYVPG LISHADLWAL AANVAIKVMG GPDIITHFGR FDCLTCNEGA QSAAG----- --RLPDGDKD ---------- --AQHLREIF CP-KGFTDKD IVALSGAHTV GACHADRS-- ---------- ---------- ---------- -GFEG--PWT DDKLKFDNSY FKDLLNKKWT C3 (A.aspera) SymAPX1 FE865713 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- C15 (M.digitata) SymAPX1 KJ672 GGEHAMAANA GLPQVAIPLL QAITEKYVPG LISHADLWAL AANVAIKVMG GPDVVTHFGR FDSLTCDEGA QSAAG----- --RLPEGDKD ---------- --AQHLREIF GP-KGFTDKD IVALSGRHTV GACHGDGS-- ---------- ---------- ---------- -GFEG--AWT DDTLKFDNSY FKDLLNKKWT D (A.hyacinthus) SymAPX1 GAFP0 GGEYAFGANA GLPQVAIPLL QAISDKYVPR LISHSDLWAL AANVAIKVMG GPDIVTHFGR FDVQAHSEGV QSAAG----- --RLPDGDKD ---------- --AKHLREIF YP-KGFTDKD IVALSGAHTV GACHGERS-- ---------- ---------- ---------- -GFEG--PWT DDKLKFDNTY FKDLLNKKWT D (A.hyacinthus) SymAPX1 GAFP0 --------GI PWLEAARGFL KAISQKYVPR LISHADLAAL AANTAIKALG GPEIPTRFGR LDIRSEDDV- ---------- --MLPDGEKD ---------- --AKYIRDVL GP-KGLEEKA MVAVLGANVC GMCHVEGA-- ---------- ---------- ---------- -DFG------ --ARRFDNSY FKDLLTKQWE F1 (Mv) SymAPX1 KJ672514 GCENGMAENA GLPQVAIPLL ASISEKYVPG LISHADLWAL AANVAIKAMG GPDIVTHFGR FDSLSAEECA KCAAG----- --RLPDGDKG ---------- --AAHLRDVF GP-KGFTDRD IVALSGAHVV WGCYPDQS-- ---------- ---------- ---------- -VFEG--VWI DDKVKFDNSY FKDLLSKKWT F1 (CCMP2468) SymAPX1 Assembly GCENGMAENA GLPQVAIPLL ASISEKYVPG LISHADLWAL A--------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- F1 (CCMP2468) SymAPX1 Assembly SGENAMGAIA GLPDVAIGLL RGISDKYVPR LISHADLWAL A--------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- F1 (CCMP2468) SymAPX1 Assembly ---------- ---------- ---------- -------AAL AANVAIRAMG GPEIPTRFGR VDVKTSAGCV PHAVG----- --RLPDGDQD ---------- --AKHIREIF GP-KGFADKD IVALSGAHTM G------S-- ---------- ---------- ---------- --FEG--PWT ASKLKFDNSY FKDLLNKKWE A1 (Casskb8) SymAPX2 Assembly1 DGEGTFGANA GLPDVALGLL KPITEKYVPD TIGHADLWTL AANVAIKVMG GPDIKTRFGR KDAESSADSV ESQVG----- --RLPDGDKE ---------- --IDHLREIF AP-KGFSDKE IVALSGAHTV GKCHLDRS-- ---------- ---------- ---------- -GFDG--PWT EEPLKFDNSY FKDMLAKEWT A1 (CCMP2467) SymAPX2 Locus_19 DGEGTFGANA GLPDVALGLL KPITEKYVPD TIGHADLWTL AANVAIKVMG GP-------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- A1 (CCMP2467) SymAPX2 KJ672516 DGEGTFGANA GLPDVALGLL KPITEKYVPD TIGHADLWTL AANVAIKVMG GPDIKTRFGR KDAESSADSV ESQVG----- --RLPDGDKE ---------- --IDHLREIF AP-KGFSDKE IVALSGAHTV GKCHLDRS-- ---------- ---------- ---------- -GFDG--PWT EEPLKFDNSY FKDMLAKEWT B1 (Mf1.05b) SymAPX2 rep_c2343 ---------- ---------- ---------- ---------- ---LAIKVMG GPDIKTRFGR KDAESSADSV ESQVG----- --RLPDG--- ---------- ----HLREIF AP-KGFSDKD IVALSGAHTV GKCHLDRS-- ---------- ---------- ---------- -GFDG--PWT EAPLKFDNSY FKDMLAKEWT C3 (A.aspera) SymAPX2 Assembly ---------- ---------- ---------- ---HADLWTL AANVAIKVMG GPDIKTRFGR KDAESSADSV ESQVG----- --RLPDGDKE ---------- --IDHLREIF AP-KGFSDKE IVALSGAHTV GKCHLDRS-- ---------- ---------- ---------- -GFDG--PWT EEPLKFDNSY FKDMLAKEWT D (A.hyacinthus) SymAPX2 GAFP0 AGEGTFGANA GLPDVALGLL KPITEKYVPS VIGHADLWTL AANVAIKEMG GPDIKTRFGR KDAESSSDSV ESQVG----- --RLPDGDKE ---------- --IDHLREIF HP-KGFDDKD IVALSGAHTV GKCHLDRS-- ---------- ---------- ---------- -GFDG--AWT EEPLKFDNTY FKEMLEKEYS D (A.hyacinthus) SymAPX2 GAFP0 AGEGTFGANA GLPDVALGLL KPITEKYVPS VIGHADLWTL AANVAIKEMG GPVIKTRFGR TDAKSSAESV ESQVG----- --RLPDGDKE ---------- --LDHLREIF HP-KGFDDRD IVALSGAHTV GKCHIGRS-- ---------- ---------- ---------- -GFDG--AWT EEPLKFDNSY FKEMLQKEYK A1 (Casskb8) SymAPX3 Assembly2 EGEMNFGANA GL-DKAKGFL DEFAKKY--P EISWADLIQM ASAVAIECAG GPAIDMKYGR VAVCCNNECV KSDSREGFGG NAGLPDAKPP ----FGCGAP TAAQHLRNVF TKKMGFTDQE IVALSGAHTI GRAFKERSGA CPFGYGEQSA SKYTKS---D CIARKDGSKG VGMVGGAAWT KNWLTFDNSY FKDYKL---- A1 (Casskb8) SymAPX3 Assembly3 AGEMNFGANA GL-DKAKGFL DEFAKKY--P EISWADLIQL ASAVAIECAG GPAIDMKYGR VAVTSDSECV KSGSREGFSG NAGLPDAKPP ----FGCGAP TAAQHLRNVF TKKMGFTDQE IVALSGAHTI GRAFKERSGA CPFGYGEQGA SKYTKS---D CLARKDGQKG VGMVGGAAWT KNWLTFDNSY FKDYKLAS-- A1 (Casskb8) SymAPX3 rep_c280 AGEMNFGANA GL-DKAKGFL DEFAKKY--H EISWADLIQL ASAVAIECAG GPAIDMKYGR VAVTSDSECV KSASREGFSG NAGLPDAEPP ----FGCGAP TAAQHLRNVF TKKMGFTDQE IVALSGAHTI GRAFKERSGA CPFGYGEQAA SKYTKG---D CIVRKDGSKG VGMVGGASWT KNWLTFDNSY FKDYKLSY-- A1 (CCMP2467) SymAPX3 Locus_31 ---------- ---------- DEFAKKY--H EISWADLIQM ASAVAIECAG GPAIDMKYGR VAVTSDSECV KSASREGFSG NAGLPDAEPP ----FGCGAP TAAQHLRNVF TKKMGFTDQE IVALSGAHTI GRAFKERSGA CPFGYGEQGA SKYTKS---D CIARKDGSKG VGIVGGAAWT KNWLTFDNSY FKDYKLAS-- A1 (CCMP2467) SymAPX3 Assembly EGEMNFGANA GL-DKAKGFL DEFAKKY--P EISWADLIQM ASAVAIECAG GPAIDMKYGR GAVTSDSECV KSGSREGFSG NAGLPDAKPP ----FGCGAP TAAQHLRNVF TKKMGFTDQE IVALSGAHTI GRAFKERSGA CP-------- ---------- ---------- ---------- ---------- ---------- D (A.hyacinthus) SymAPX3 GAFP0 DPEMNMGANA GL-DKARGYL ETIQKNH--P SVSWADLIQL ASATAIEMAG GPVIKMKYGR VSVTDPSQCA GPASREGFKG NAGLPDAAPP ----FGCGAQ KPEEHLRNVF GKKMGFTDQE IVALSGAHTL GRVFKERSGA CPFGYGEQSA SKYTKG---N CIARKDGKSG VGMVGGGAWT KNWLTFDNSY FANYKDAM-- B1 (Mf1.05b) SymAPX4 Assembly2 EPEISYGANA GL-TKAVNFL KAFKQKY--P GITWADLIQM ASACAIQATG GPILPMKYGR VDVKDGSGCP GRTSR-GTAD NAGLPDAMAP ----FGCGAQ DPATHLRNIF NR-MGFDDEG IVALSGAHTL GRAFQERSXV VENGYGXAKA STYTKS---G CPVRHDGKGG VGMPGGKSWT PNWLTFDNSY FQFLKRS--- D (A.hyacinthus) SymAPX4 GAFP0 EPEISYGANA GL-TKAVNFL KPFKQKY--A AVSWADLIQM ASACAIQVTG GPILPMKYGR VDVKDGSACP GRTSR-GTAD NAGLPDAMAP ----FGCGAK QPATHLRNIF HR-MGFDDEG IVALSGAHTL GRAFQERSGV VENGYGDAKA SPYTKS---G CPVRHDGKAG FGMPGGKAWT SNWLTFDNSY FQFLKRS--- F1 (CCMP2468) SymAPX4 KC944388 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------D NAGLPDAMAP ----FGCGAQ DPQTHLRNIF NR-MGFDDEG IVALSGAHTL GRAFQERSGV VQNGYGDAKA SSHTKS---G CPVRHDGKAG VGMPGGMAWT PNWLTFDNSY FQFLKRA--- A1 (Casskb8) SymAPX5 rep_C1877 SPEITMGANN GL-DKAVKYL EPFKADY--P LVSYADLYQM AAAVSIQHAG GPEIEMKYGR KDATGPEACP GRQSR-GTAD NAGLPDAEPG PEGKFGCGAT DAASHLRNIF YR-MGFDDKG IVALSGAHTI GRAFKERSGT VKEGYGESNG CPYTRALPAS CPIRHDGKGG VGMPGGKSWT SKWLKFDNEY FQPSVYEE-- A1 (CCMP2467) SymAPX5 Locus_10 SPEITMGANN GL-DKAVKYL EPFKADY--P LVSYADLYQM AAAVSIQHAG GPEIEMKYGR KDATGPEACP GRQSR-GTAD NAGLPDAEPG PEGKFGCGAT DAASHLRNIF YR-MGFDDKG IVALSGAHTI GRAFKERSGT VKEGYGESNG CPYTRALPAS CPIRHDGKGG VGMPGGKSWT SKWLKFDNEY FQPSVYEE-- D (A.hyacinthus) SymAPX5 Assem SPEISMGANN GL-DKGVKYL EPFKADY--P LVSYADLYQM AAAVSIEHAG GPKIDMKYGR KDASGPDDCP GRQSR-GTAD NAGLPDAEPG PDGAFGCGAK DAATHLRNIF YR-MGFDDKG IVALSGAHTI GRAFKERSGT VKEGYGESSA CPYTRSMPKG CPIRNDGGYG VGMPGGKSWT TKWLKFDNEY FQPSVYEE-- A1 (CCMP2467) SymAPX6 Locus_36 ELMRDENYGP SM-AAALAAV ESIVQR---C KVSWADAIAV SGAAVVEAMG GPVIPVETGR SDATEPD--- ---------T DKRLPDGTLT ---------- --SGAIRDYF RQ-LGMSDAE LVALLGAHTV GRWTSLLDVR PDCLHKEGRA FWTCSR---- ---------- -EQGRRLAFT ESPAAFNNEY FQEVLLWEQR B1 (Mf1.05b) SymAPX6 rep_c6573 ELMRNENIGP SM-RQALQFI EQIVQN---C QVSWADAIAV SGAAVVEALG GPKIDVATGR KDALEED--- ---------A ENRLPDGSLT ---------- --SGAIRDYF RQ-LGFSDEE LVALVGAHTI GRWTSLLGVQ PACMAKEGLD FWICTR---- ---------- -EQGQRLPFT ARPTLFNNDY FKNVLEFERR A1 (Casskb8) APX Assembly4 EPEMTMGANA GL-DKAKRYL EAFAKKY--P LISWADLIQL ASATAVEVTG GPVIDMVYGR VAVAGPQDCV GATSRQGFAG NAGLPDALPP ----FGCGAT TPAEHLRNVF TKKMGFNDQE IVALSGAHTV GRAFKERSGA CPFGYGDASA SKHTKS---T CKVRKDNADG VGMAGGCPWT KNWLTFDNSY FSRYKDAM-- A1 (CCMP2467) APX Locus_35678 EPEMTMGANA GL-DKAKRYL EAFAKKY--P LISWADLIQL ASATAVEVTG GPVIDMVYGR VAVAGPQDCV GATSRQGFAG NAGLPDALPP ----FGCGAT TPAEHLRNVF TKKMGFNDQE IVALSGAHTV GRAFKERSGA CPFGYGDASA SKHTKS---T CKVRKDNADG VGMAGGCPWT KNWLTFDNSY FSRYKDAM-- ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| .... 405 415 425 435 445 455 465 475 A1 (Casskb8) SymAPX1 rep_c1302 METVKSGNKQ Y------RSG KEMMLTTDMA LVEDPKFKEF VVKYASNQGA FFDDFKEAWV KLQ------- ---------- ---- A1 (CCMP2467) SymAPX1 GAKY0104 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---- A1 (Casskb8) SymAPX1 rep_c1797 KETNRHGKPQ Y------RSG ETMMLTTDMA LVEDPAFKQH VERYAADQKS WFDDFAKAWV RLQEFNSGEL RDIL*----- ---- A1 (CCMP2467) SymAPX1 Locus_98 KETNRHGKPQ Y------RSG ETMMLTTDMA LVEDPAFKQH VERYAADQKS WFDDFAKAWV RLQEFNSGEL RDIL*----- ---- A1 (Casskb8) SymAPX1 rep_c1337 SETTSKGNPQ FRC----AES KTMMLTTDMA LVNDPDFKRY VIQFADDQKS WFSEFDKAWV RLQELGCSEL RDVL*----- ---- A1 (CCMP2467) SymAPX1 Locus_51 SETTSKGNPQ FRC----AES KTMMLTTDMA LVNDPDFKRY VIQFADDQKS WFSEFDKAWV RLQELGCSEL RDVL*----- ---- A1 (CCMP2467) SymAPX1 Assembly METVKSGNKQ Y------RSG KEMMLTTDMA LVEDPKFKEF VVKYASNQGA FFDDFKEAWV KLQELGCGQL RDIL*----- ---- B1 (Mf1.05b) SymAPX1 Assembly1 PETRKSGKKQ Y------RNG KTMMLPSDMA LIEDSKFKEY VEKYASDQQA FFVDFVEAWT RLQELGCGQL RDTL*----- ---- B1 (Mf1.05b) SymAPX1 rep_c960 KETNAKGKVQ Y------KCG DTMMLTTDIA LIEDEAFKVH VQKYADDRQL WFDDFTKAWV KLQEFGTKDL RDIL*----- ---- B1 (Ap1) SymAPX1 KJ672513 FQTVKSGNKQ Y------MSG KTMMLTTDMA LIEDAKFKEY VQKYAKDQGA ---------- ---------- ---------- ---- B1 (Mf1.05b) SymAPX1 Assembly3 FQTVKSGNKQ Y------MSG KTMMLTTDMA LIEDAKFKEY VQKYAKDQGA FFADYKDAWV RLQELGCGQL RDTL*----- ---- B1 (Mf1.05b) SymAPX1 rep_c3008 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---- B1 (Mf1.05b) SymAPX1 Assembly4 EETTS----- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---- C1 (CCMP2466) SymAPX1 KF835562 LETMKPGKPQ Y------WSG KTMMLTTDMA LVEDAKFKEH V--------- ---------- ---------- ---------- ---- C3 (S.hystrix) SymAPX1 HM15669 LETLKPGKPQ Y------WSG KTMMLTTDMA LVEDAKFKEH VQKYANDQEA FFQDFVEAWV RLQELGCGQL RDIL*----- ---- C3 (A.aspera) SymAPX1 FE865713 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---- C15 (M.digitata) SymAPX1 KJ672 LETMKSGKKQ Y------WSG KTMMLPTDMA LVLDAKFK-- ---------- ---------- ---------- ---------- ---- D (A.hyacinthus) SymAPX1 GAFP0 METVKSGNKQ Y------RSG KTMMLTTDMC LIEDPKFKEY AVQYA----- ---------- ---------- ---------- ---- D (A.hyacinthus) SymAPX1 GAFP0 QETSQNGTPQ F------RCG DAVMMSTDMA LVND------ ---------- ---------- ---------- ---------- ---- F1 (Mv) SymAPX1 KJ672514 TETGKSGKKQ Y------RNG NAMMLPSDIA LIEDPKFKQH VEKYAGDQAA FFADFKEAWV RGQE------ ---------- ---- F1 (CCMP2468) SymAPX1 Assembly ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---- F1 (CCMP2468) SymAPX1 Assembly ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---- F1 (CCMP2468) SymAPX1 Assembly KETNSKGKIQ Y------RCG DTMMLTTDMA FIEDESFKVH VEKYAADQKL WFEDFTKAWV SLQEFGLEEL REIL*----- ---- A1 (Casskb8) SymAPX2 Assembly1 DDKTEKGNPQ FK-----SES GTIMLISDLA LLKDPAFKEH VEKYAADQDA YFADFTTAWV KLQENGCSGL RDSL*----- ---- A1 (CCMP2467) SymAPX2 Locus_19 ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---- A1 (CCMP2467) SymAPX2 KJ672516 DDKTEKGNPQ FK-----SES ---------- ---------- ---------- ---------- ---------- ---------- ---- B1 (Mf1.05b) SymAPX2 rep_c2343 DDKTEKGNPQ FK-----SES GTIMLISDLA LLKDPAFKEH VEKYAADQDA YFADFTTAWV KLQENGCSGL RDSL*----- ---- C3 (A.aspera) SymAPX2 Assembly DDKTEKGNPQ FK-----SES GTIMLISDLA LLKDPAFKEH VEKYAADQDA YFADFTTAWV KLQENGCSDL RDSL*----- ---- D (A.hyacinthus) SymAPX2 GAFP0 EDKTEKGNPQ FK-----SGS GTIMLISDLA LLKDSAFKKH VETYAKDQDA YFKDFTTAWV KLQENGVTGL RDSL*----- ---- D (A.hyacinthus) SymAPX2 GAFP0 EEKTEKGNPQ FK-----SGS GTIMLTSDLA LLKDPAFRQH VEAYAKDQEV FFKDFATAWI KLQENGVAGL RDQL*----- ---- A1 (Casskb8) SymAPX3 Assembly2 ---------- -------SDQ NLLWFPTDEA LHTDPEFKKY FELYAKDQDA FFKDYAKAHK KLSELGAKFE PAQGITI*-- ---- A1 (Casskb8) SymAPX3 Assembly3 ---------- -------EDE NLLWFPTDEA LHTDPEFKKY FDIYAKDQDA FFKDYAKAHK KLSELGAKFE PEQGIKI*-- ---- A1 (Casskb8) SymAPX3 rep_c280 ---------- -------EDD SLLWFKTDEA LHTDPEFKKY FDLYAKDQDA FFKDYAKAHK KLSELGSKFE PEGGIKI*-- ---- A1 (CCMP2467) SymAPX3 Locus_31 ---------- -------EDE NLLWFPTDEA LHTDPEFKKY FELYAKDQDA FFKDYAKAHK KLSELGAKFE PEQGIKI*-- ---- A1 (CCMP2467) SymAPX3 Assembly ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---------- ---- D (A.hyacinthus) SymAPX3 GAFP0 ---------- -------DDD DLIWLPTDEV LHNDPEFKKY FYKYAGDQNA FFQDMALAHK KLSELGAKFD PPSGFEI*-- ---- B1 (Mf1.05b) SymAPX4 Assembly2 ---------- -------KDE NLLWLPTDAA LHEDPKFKVY FDRFAESKEA FFEAYSKAHT KLSELGSKFE PAEGFSLPPA SKL* D (A.hyacinthus) SymAPX4 GAFP0 ---------- -------KDE NLLWLPTDAA LHEDPQFKVY FDRFADSKDA FFDAYSKAHT KLSELGSKFE PAGGFSLPPA SKL* F1 (CCMP2468) SymAPX4 KC944388 ---------- -------KDD NLLWLPTDAA LHEDPKFKVY FDRFAESKEA FFEAYSKAHT KLSELGSKFE PAEGFSLPPA SKL* A1 (Casskb8) SymAPX5 rep_C1877 ---------- -------KDA NLLWLSSDRC LHQDESFKKY FMQYRDDQAA FFKDFAEAYK QLSEQGAEFE PAGGISI*-- ---- A1 (CCMP2467) SymAPX5 Locus_10 ---------- -------KDA NLLWLSSDRC LHQDESFKKY FMQYRDDQAA FFKDFAEAYK QLSEQGAEFE PAGGISI*-- ---- D (A.hyacinthus) SymAPX5 Assem ---------- -------KDK DLLWLSSDRC LHQDEGFKQY FMLYKDDQAA FFRDFAEAYK QLSEQGAEFE PEAGLTL*-- ---- A1 (CCMP2467) SymAPX6 Locus_36 QKMPKPKTRY KERAESEKSS PLNLLPSDIG MLFDPGLHSQ VERFAADEAA FFAAFASGYR KLVN*----- ---------- ---- B1 (Mf1.05b) SymAPX6 rep_c6573 QKMPRPTKRY KDRPENGGLP ALYLLPSDIG LLYDADLHKQ VEKFAFDEAA FFAAFSRAYL KLVT*----- ---------- ---- A1 (Casskb8) APX Assembly4 ---------- -------ADD ELLWFPTDEA LHTDPGFKPH FDKYAADQNA FFADYARAHK KLSELGTKFE PQGGV----- ---- A1 (CCMP2467) APX Locus_35678 ---------- -------ADD NLLWFPTDEA LHTDPGFKPH FD------NS YFS------- ---------- ---------- ----