Growth Cone Localization of the mRNA Encoding the Chromatin Regulator HMGN5 Modulates Neurite Outgrowth

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 -

  Legends to Movies S1 to S5; Fig. S1 (Validation of effectiveness of EHNA treatment), S2 (Transcriptomic analysis of Hmgn5 KD N1E-115 cells), S3 (mRNA localization and protein expression of HMGN5-GFP constructs in N1E-115 cells), S4 (Chromatin analysis of HMGN5-GFP expression constructs), S5 (mRNA localization of HMGN5-GFP constructs in hippocampal neurons), S6 (Axonal specification in Hmgn5 KD hippocampal neurons) and Table S1 (Primers)

  PDF, 7.8M

• Supplemental file 2 -

  Movie S1 (Growth cone mRNA translation using PalX2-Dendra2 reporters)

  MOV, 451K

• Supplemental file 3 -

  Movie S2 (Anterograde and retrograde transport in N1E-115 cells treated with vehicle)

  AVI, 1020K

• Supplemental file 4 -

  Movie S3 (Anterograde and retrograde transport in N1E-115 cells treated with EHNA)

  AVI, 1.2M

• Supplemental file 5 -

  Movie S4 (Neurite outgrowth dynamics of control and Hmgn5 KD N1E-115 cells)

  MOV, 6.1M

• Supplemental file 6 -

  Movie S5 (Histone H1 FRAP analysis)

  MOV, 130K