Fermentation and Cost-Effective ¹³C/¹⁵N Labeling of the Nonribosomal Peptide Gramicidin S for Nuclear Magnetic Resonance Structure Analysis

Supplemental material

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• Supplemental file 1 -

  Supplemental methods (chromatographic purification of GS and determination of the extent of labeling, solid-state nuclear magnetic resonance, dihedral angle prediction); ¹³C solid-state chemical shifts of GS given with respect to TMS (δ_TMS) and DSS (δ_DSS) and corresponding characteristic chemical shift regions as provided by the Biological Magnetic Resonance Data Bank (Table S1); dihedral angles in the GS backbone as predicted by the TALOS+ program (Table S2); two-dimensional ¹³C-¹³C PARIS spectrum of membrane-bound GS with a mixing time of 35 ms (Fig. S1).

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