Lantibiotic Reductase LtnJ Substrate Selectivity Assessed with a Collection of Nisin Derivatives as Substrates

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  MS spectrum of peptide purified from NZ9000(pIL3EryBTC; pNZ-nisA-T-ltnJ) showing that part of the peptide was modified by LtnJ (Fig. S1), MS/MS spectra and amino acid sequences of the C terminus of L27F (Fig. S2), I25F (Fig. S3), L27N (Fig. S4), L27A (Fig. S5), I25A-L27A (Fig. S6), and I25A (Fig. S7) released after tryptic digestion containing D-alanine, and comparison of the LC-MS profiles of L27A tail expressed with/without LtnJ when threonine30 was not dehydrated (Fig. S8).

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