Rab27a controls HIV-1 assembly by regulating plasma membrane levels of phosphatidylinositol 4,5-bisphosphate

Files in this Data Supplement:

• Supplemental Material (PDF)
• Video 3 -
  Localization and dynamics of mCherry-CD63 and GFP-PI4KIIα in Slp2a-silenced Jurkat cells. Time-lapse confocal microscopy of live Slp2a-silenced Jurkat cells expressing both mCherry-CD63 (red) and GFP-PI4KIIα (green). Images were acquired using a laser-scanning confocal microscope (spectral FV 1000; Olympus). Frames were taken every 1.325 s for 2.5 min. The video plays at 6 fps. Bar, 2 µm. Selected frames from this video are shown in Fig. S5 B.
• Video 1 -
   Localization and dynamics of mCherry-CD63 and GFP-PI4KIIα in control Jurkat cells. Time-lapse confocal microscopy of live control Jurkat cells expressing both mCherry-CD63 (red) and GFP-PI4KIIα (green). Images were acquired using a laser-scanning confocal microscope (spectral FV 1000; Olympus). Frames were taken every 1.325 s for 2.5 min. The video plays at 6 frames per s (fps). Bar, 2 µm. Selected frames from this video are shown in Fig. S5 B. Arrows point to double-positive vesicles with long-range movements.
• Video 2 -
   Localization and dynamics of mCherry-CD63 and GFP-PI4KIIα in Rab27a-silenced Jurkat cells. Time-lapse confocal microscopy of live Rab27a-silenced Jurkat cells expressing both mCherry-CD63 (red) and GFP-PI4KIIα (green). Images were acquired using a laser-scanning confocal microscope (spectral FV 1000; Olympus). Frames were taken every 2.65 s for 3.7 min. The video plays at 3 fps. Bar, 2 µm. Selected frames from this video are shown in Fig. S5 B.