Fig. S1. V. cholerae induces activation of NF-κB independent of priming with E. coli LPS and does not depend on secreted toxins. Fig. S2. ASCdef cells do not produce ASC. Fig. S3. El Tor and classical strains grow equally well. Fig. S4. LPS alone does not stimulate IL-1β secretion from THP-1 cells. Fig. S5. ΔhlyA mutants do not secrete hemolysin. Fig. S6. NLRP3def THP-1 cells do not respond to LPS in the presence of 2 mM CaCl2. Fig. S7. V. cholerae induces production of pro-IL-1β and processing of caspase 1. Fig. S8. V. cholerae induces secretion of TNF-α independent of toxins. Fig. S9. V. cholerae but not forskolin induces IL-1β secretion.
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