Analysis of the Effects of Cigarette Smoke on Staphylococcal Virulence Phenotypes

Supplemental material

  • Supplemental file 1 -

    Fig. S1. MRSA exposed to CSE prior to incubation with human AMP LL-37 had a higher MBC compared to control MRSA (32 μM vs 8 to 16 μM, respectively), as evidenced by live bacteria growing on THA plates after overnight incubation with LL-37. Table S2. Compared to control MRSA, CSE-MRSA were more resistant to LL-37 by MIC, metabolic activity, and MBC assays. Fig. S3. S. aureus strain SA113ΔmprF, in which mprF is knocked out, had no change in PLL-FITC binding after exposure to CSE.

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