Biosynthesis of the Common Polysaccharide Antigen of Pseudomonas aeruginosa PAO1: Characterization and Role of GDP-d-Rhamnose:GlcNAc/GalNAc-Diphosphate-Lipid α1,3-d-Rhamnosyltransferase WbpZ

Supplemental material

• Supplemental file 1 -

  Supplemental text

  Table S1, sequence identities of GT4 family glycosyltransferases

  Table S2, primers

  Fig. S1, multiple-sequence alignment of WbpZ with other bacterial transferases

  Fig. S2, expression and purification of WbpZ

  Fig. S3, effect of detergent on d-Rha-transferase activity

  Fig. S4, dependence of d-Rha-transferase activity on buffer pH

  Fig. S5, metal ion requirement of d-Rha-transferase activity

  Fig. S6, kinetics of d-Rha-transferase activity

  Fig. S7, TOCSY NMR spectrum for d-Rha-transferase product

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