Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie 4 - Video 4. Time lapse analysis of GFP Mlc1 and Cdc3 mCherry at the bud neck during the cell cycle in cdc12 6 myo1Δ cells (YEF7081) at 39°C in the absence (left) or presence (right) of LatA (related to Figure 2, C and D). 2 min interval. Scale bar, 2 μm.
- Movie 7 - Video 7. Time lapse analysis of GFP Mlc1 and Cdc3 mCherry at the bud neck during the cell cycle in myo1Δ cells (YEF7132) at 25°C in the absence (left) or presence (right) of LatA (related to Figure 5A). 2 min interval. Scale bar, 2 μm.
- Movie 1 - Video 1. Time lapse analysis of GFP Mlc1 and Cdc3 mCherry at the bud neck during the cell cycle in cells expressing GFP Mlc1 as the sole source of Mlc1 (YEF6800) at 25°C (related to Supplemental Figure S1). 3 min interval. Scale bar, 2 m.
- Movie 5 - Video 5. Time lapse analysis of GFP Mlc1 and Cdc3 mCherry at the bud neck during the cell cycle in WT cells (YEF7435) at 25°C in the absence (left) or presence (right) of LatA (related to Figure 3A). 2 min interval. Scale bar, 2 μm.
- Movie 6 - Video 6. Time lapse analysis of GFP Mlc1 and Cdc3 mCherry at the bud neck during the cell cycle in bnr1Δ (YEF7200) (left) or bni1Δ cells (YEF7201) (right) at 25°C (related to Figure 4A). 2 min interval. Scale bar, 2 μm.
- Movie 9 - Video 9. Time lapse analysis of GFP Mlc1 and Cdc3 mCherry at the bud neck during the cell cycle in myo1Δ myo2IQ6Δ myo4Δ cells (YEF7055) at 25°C in the absence (left) or presence (right) of LatA (related to Figure 6C). 2 min interval. Scale bar, 2 μm.
- Movie 2 - Video 2. Time lapse analysis of GFP Mlc1 and Cdc3 mCherry at the bud neck during the cell cycle in cdc12 6 cells (YEF6884) at 39°C in the absence (left) or presence (right) of LatA (related to Figure 1, B and D). 2 min interval. Scale bar, 2 μm.
- Movie 8 - Video 8. Time lapse analysis of GFP Mlc1 and Cdc3 mCherry at the bud neck during the cell cycle in bni5Δ cells (YEF7259) at 25°C (Supplemental Figure S2). 2 min interval. Scale bar, 2 μm.
- Movie 10 - Video 10. Time lapse analyses of GFP Mlc1 Nterm and Cdc3 mCherry at the bud neck during the cell cycle in WT cells (YEF7208) at 25°C (first column from the left), and of GFP Mlc1 Cterm and Cdc3 mCherry at the bud neck during the cell cycle in WT (YEF7209) (second column), myo2IQ6Δ (YEF7245) (third column), and LatA treated WT (YEF7209) (last column) cells at 25°C (related to Figure 7, B E). 2 min interval. Scale bar, 2 μm.
- Movie 3 - Video 3. Time lapse analysis of Myo1 GFP and Cdc3 mCherry at the bud neck during the cell cycle in cdc12 6 cells (YEF7155) at 39°C in the absence (left) or presence (right) of LatA (related to Figure 2, A and B). 2 min interval. Scale bar, 2 μm.