Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie 3 - Movie S3. 360° rotation of an electrostatic surface representation of the PICK1 model. Red: negatively charged, blue: positively charged. Movie related to Figure 2E.
- Movie 2 - Movie S2. 360° rotation of the atomic model of PICK1. The PICK1 domains are color-coded according to Figure 2A. The side chains of positively-charged amino acids predicted to participate in membrane binding are shown. Movie related to Figure 2C.
- Movie 1 - Movie S1. 360° rotation of the MBP-PICK1 model inside the SAXS envelope. The PICK1 domains are color-coded according to Figure 2A, and MBP is shown in green. Movie related to Figure 2B.
- Movie 7 - Movie S7. B16F1 cells expressing GFP-PICK1129-415 with or without treatment with latrunculin B. Each movie consists of 15 frames, taken at 4 s intervals, for a total of 60 s, and played at 10x their actual speeds. Movies related to Figure 4C.
- Movie 8 - Movie S8. HeLa cells expressing GFP-PICK1129-415 with or without treatment with CK-666. Each movie consists of 20 frames, taken at 3 s intervals, for a total of 60 s, and played at 10x their actual speeds. Control cells were treated with DMSO. Movies related to Figure 6.
- Movie 4 - Movie S4. HeLa cells expressing GFP-PICK1 constructs. From left to right the movies show cells expressing constructs GFP-PICK1, GFP-PICK1129-415, GFP-PICK11-375, and GFP-PICK1129-375 (as indicated). Each movie consists of 20 frames, taken at 3 s intervals, for a total of 60 s, and played at 10x their actual speeds. Movies related to Figure 3B.
- Movie 6 - Movie S6. HeLa cells coexpressing GFP-PICK1129-415 (green) and RFP-actin (red). The movie consists of 20 frames, taken at 3 s intervals, for a total of 60 s, and is played at 10x its actual speed. Movies related to Figure 4B.
- Movie 5 - Movie S5. HeLa cells coexpressing PICK1 constructs and the trans-Golgi marker TGN38. From left to right the movies show cells coexpressing constructs mCherry-PICK1129-415, mCherry- PICK11-375, and mCherry-PICK1129-375 (red) with CFP-TGN38 (green). Each movie consists of 20 frames, taken at 2 s intervals, for a total of 40 s, and played at 5.7X their actual speeds. Movies related to Supplemental Figure S3.